Compositions and methods of application of reactive antiviral polyadenylic acid derivatives

• US 5,496,546 A
• Filed: 02/23/1994
• Issued: 03/05/1996
• Est. Priority Date: 02/24/1993
• Status: Expired due to Term

• Current Assignee: Alumiplate, Inc., DNP Pharmaceuticals, Inc.
• Original Assignee: Jui H. Wang
• Inventors: Rahman, Mohammed H., Kang, Insug, Wang, Jui H.
• Primary Examiner(s): Kulkosky, Peter F.
• Application Number:
  US08/200,650
• Time in Patent Office: 741 Days
• Field of Search: 424/78.02, 424/78.36, 424/78.38
• US Class Current: 424/78.36
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Novel polyadenylic acid (5'"'"') derivatives with 2'"'"'-0-(3-fluoro-4,6-dinitrophenyl) groups and/or 2'"'"'-0-(2,4-dinitrophenyl) groups have been synthesized and discovered to act as mutation-insensitive and function-specific inhibitors of viral reverse transcriptase. The compositions, preparative procedures and methods of application of these novel compounds for the treatment of humans carrying or infected with AIDS virus and other RNA-viruses, of other mammals carrying RNA-viruses, for the fast but temporary protection of uninfected humans and other mammals against immuno-deficiency viruses and other RNA-virus caused diseases, for the preparation of a formulation containing irreversibly sterilized HIV or other RNA-viruses useful as anti-AIDS and anti-other RNA-virus disease vaccines, for the complete sterilization of possible trace amounts of live HIV and other RNA-viruses in stored transfusion blood, and for the inactivation or removal of trace amounts of ribonuclease in solution and containers used in biotechnology processes have all been disclosed.

Claims

1. A composition of matter comprising Y-poly[A] representable by the formula:

space="preserve" listing-type="equation">M.sub.n (Y).sub.m X.sub.i[A].sub.n

where;

[A]n=polyadenylic acid (5'"'"') with n adenylic acid residues, n being at least about 20,

Y=FDNP, DNP or partially FDNP and partially DNP,

FDNP=3-fluoro-4,6-dinitrophenyl groups, attached covalently to m of the n 2'"'"'-OH groups of [A]_n via ether linkage,

DNP=2,4-dinitrophenyl groups, attached covalently to m of the n 2'"'"'-OH groups of [A]_n via ether linkage,

X=an acyl group,

i=0 or 1,

M=a cation selected to provide a desired degree of solubility for the composition,

and has one of the generic structures;

##STR4## and having a ratio of Y groups to adenine units of at least about 1;

5 and wherein n is sufficiently large so that the Y-poly[A] can substantially completely fill the active site cleft of an RNA-virus RT to effectively inactivate reverse transcriptase and/or can effectively inactivate ribonuclease.

2. A composition of matter as set forth in claim 1, wherein Y is partially or all FDNP.

3. A composition of matter as set forth in claim 1, wherein Y is partially or all DNP.

4. A composition of matter as set forth in claim 1, wherein n is at least about 25.

12. A method of temporarily protecting a healthy mammal from an RNA-virus infection comprising administering to the mammal an effective preventative amount of Y-poly[A] as defined in claim 1.

13. A method as set forth in claim 12, wherein the administering comprises parenteral injection of a sterile injectable formulation containing the Y-poly[A].

14. A method as set forth in claim 12, wherein the administration comprises the oral administration of Y-poly[A].

15. A method as set forth in claim 12, wherein the RNA-virus is HIV and the disease is acquired immuno-deficiency syndrome (AIDS).

16. A method as set forth in claim 12, wherein the RNA-virus is HIV, HTLV, HAV, HCV, influenza-viruses, parainfluenza viruses, RSV, coronavirus or rhinovirus, measles virus or mumps virus and the disease is, respectively, acquired immuno-deficiency syndrome (AIDS), adult T-cell leukemia/lymphoma, hepatitis A, hepatitis C., influenza, parainfluenza, infant bronchiolitis and infant pneumonia, common cold, measles or mumps.

17. A method as set forth in claim 12, wherein Y is partially or all FDNP.

18. A method as set forth in claim 12, wherein Y is partially or all DNP.

5. A method of treating a mammal having a disease caused by an RNA-virus comprising the administration to the mammal of an effective treatment amount of Y-poly[A].

6. A method as set forth in claim 5, wherein the administering comprises parenteral injection of a sterile injectable formulation containing the Y-poly[A].

7. A method as set forth in claim 5, wherein the administration comprises the oral administration of Y-poly[A].

8. A method as set forth in claim 5, wherein the RNA-virus is HIV and the disease is acquired immuno-deficiency syndrome (AIDS).

9. A method as set forth in claim 5, wherein the RNA-virus is HIV, HTLV, HAV, HCV, influenza-viruses, parainfluenza viruses, RSV, coronavirus or rhinovirus, measles virus or mumps virus and the disease is, respectively, acquired immuno-deficiency syndrome (AIDS), adult T-cell leukemia/lymphoma, hepatitis A, hepatitis C., influenza, parainfluenza, infant bronchiolitis and infant pneumonia, common cold, measles or mumps.

10. A method as set forth in claim 5, wherein Y is partially or all FDNP.

11. A method as set forth in claim 5, wherein Y is partially or all DNP.