Solid medium and method for DNA storage
First Claim
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1. A dry solid medium for storage of a sample of DNA comprising:
- (a) a cellulose based solid matrix; and
(b) a composition consisting essentially of a weak base, a chelating agent, an anionic surfactant or anionic detergent and optionally uric acid or a urate salt,wherein said composition is adsorbed on or incorporated into said solid matrix.
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Abstract
A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.
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Citations
16 Claims
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1. A dry solid medium for storage of a sample of DNA comprising:
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(a) a cellulose based solid matrix; and (b) a composition consisting essentially of a weak base, a chelating agent, an anionic surfactant or anionic detergent and optionally uric acid or a urate salt, wherein said composition is adsorbed on or incorporated into said solid matrix. - View Dependent Claims (2, 3, 4, 5, 13)
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6. A method for storage of a sample of DNA, and subsequent treatment of said sample of DNA comprising:
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(a) applying a sample of DNA to a dry solid medium, said dry solid medium comprising a cellulose based matrix and a composition consisting essentially of a weak base, a chelating agent, an anionic surfactant or anionic detergent and optionally uric acid or a urate salt, wherein said composition is adsorbed on or incorporated into said solid matrix; (b) storing said DNA applied to said dry solid medium; and
either;(c) treating said DNA in situ on said dry solid medium;
or(d) removing said DNA from said dry solid medium for subsequent treatment. - View Dependent Claims (7, 8, 9, 10, 11, 12, 14)
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15. A dry solid medium for storage of a sample of DNA comprising:
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(a) a cellulose-based solid matrix; and (b) a composition consisting essentially of; (i) ethylene diamine tetraacetic acid; (ii) tris-hydroxymethyl aminomethane; (iii) sodium dodecyl sulfate; and
optionally(iv) uric acid or a urate salt, wherein said composition is adsorbed on or incorporated into said solid matrix. - View Dependent Claims (16)
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Specification