Solid medium and method for DNA storage

• US 5,496,562 A
• Filed: 11/30/1993
• Issued: 03/05/1996
• Est. Priority Date: 10/05/1988
• Status: Expired due to Term

• Current Assignee: Whatman Ltd. (United Kingdom) (General Electric Co. (Italy))
• Original Assignee: Flinders Technologies Pty., Ltd.
• Inventors: Burgoyne, Leigh A.
• Primary Examiner(s): Zitomer, Stephanie W.
• Application Number:
  US08/159,104
• Time in Patent Office: 826 Days
• Field of Search: 435/6, 435/91, 435/91.2, 424/443, 424/464, 424/484-488, 424/489, 424/490, 424/499
• US Class Current: 424/488
• CPC Class Codes: C12N 15/1006 by means of a solid support carrier, e.g. particles, polymers
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6841 In situ hybridisation
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 1/6879 for sex determination
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574

Abstract

A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.

Claims

1. A dry solid medium for storage of a sample of DNA comprising:

(a) a cellulose based solid matrix; and

(b) a composition consisting essentially of a weak base, a chelating agent, an anionic surfactant or anionic detergent and optionally uric acid or a urate salt,

wherein said composition is adsorbed on or incorporated into said solid matrix.

2. The dry solid medium according to claim 1, wherein said sample of DNA is in whole blood.

3. The dry solid medium according to claim 1, wherein said composition further consists essentially of uric acid or a urate salt.

4. The dry solid medium according to claim 1, wherein said composition protects against degradation of DNA.

5. The dry solid medium according to claim 1, further comprising a sample of DNA applied thereto, and a polystyrene material impregnating or encasing said medium and said sample of DNA.

13. The dry solid medium according to claim 1, wherein said sample of DNA is semi-purified or purified DNA.

6. A method for storage of a sample of DNA, and subsequent treatment of said sample of DNA comprising:

(a) applying a sample of DNA to a dry solid medium, said dry solid medium comprising a cellulose based matrix and a composition consisting essentially of a weak base, a chelating agent, an anionic surfactant or anionic detergent and optionally uric acid or a urate salt, wherein said composition is adsorbed on or incorporated into said solid matrix;

(b) storing said DNA applied to said dry solid medium; and

either;

(c) treating said DNA in situ on said dry solid medium;

or

(d) removing said DNA from said dry solid medium for subsequent treatment.

7. The method according to claim 6, wherein the composition further consists essentially of uric acid or a urate salt.

8. The method according to claim 6, wherein said sample of DNA is in whole blood.

9. The method according to claim 6, wherein said composition protects against degradation of DNA.

10. The method of claim 6, wherein treating said stored sample of DNA in situ on said dry solid medium further comprises a step of removing proteins contained in said stored sample of DNA, said step of removing proteins comprising:

(a) contacting said sample of DNA stored on said dry solid medium with a one-phase phenol solution;

(b) removing said one-phase phenol solution from said sample of DNA stored on said dry solid medium;

(c) contacting said sample of DNA stored on said dry solid medium with an aqueous alcohol solution; and

(d) removing said aqueous alcohol solution from said sample of DNA stored on said dry solid medium,

to provide an essentially protein free stored sample of DNA.

11. The method according to claim 6, further comprising the step of DNA sequence amplification by polymerase chain reaction.

12. The method according to claim 6, wherein said step of storing said DNA applied to said dry solid medium further comprises applying a polystyrene material impregnating or encasing said dry solid medium after applying said sample of DNA.

14. The method according to claim 6, wherein said sample of DNA is semi-purified or purified DNA.

15. A dry solid medium for storage of a sample of DNA comprising:

(a) a cellulose-based solid matrix; and

(b) a composition consisting essentially of;

(i) ethylene diamine tetraacetic acid;

(ii) tris-hydroxymethyl aminomethane;

(iii) sodium dodecyl sulfate; and

optionally

(iv) uric acid or a urate salt,

wherein said composition is adsorbed on or incorporated into said solid matrix.

16. The dry solid medium according to claim 15, further comprising a sample of DNA applied thereto, and a polystyrene material impregnating or encasing said medium and said sample of DNA.