Reagent composition for quantitative analysis of inorganic phosphorous and dry analysis element utilizing the same

• US 5,496,708 A
• Filed: 06/14/1994
• Issued: 03/05/1996
• Est. Priority Date: 06/15/1993
• Status: Expired due to Term

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Yamada, Kiyoshi, Amano, Yoshikazu, Kawasaki, Kazuya, Seshimoto, Osamu
• Primary Examiner(s): Gitomer, Ralph J.
• Application Number:
  US08/259,616
• Time in Patent Office: 630 Days
• Field of Search: 435/25, 435/28, 435/805, 435/969, 435/970, 422/56, 422/57, 436/170, 436/810, 544/268
• US Class Current: 435/28
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 435/97 Test strip or test slide

Abstract

A reagent composition for the quantitative analysis of inorganic phosphorus, composted of xanthosine, purine nucleoside phosphorylase, xanthine oxidase and a color-former. The color-former is a precursor for forming a dye in the presence of hydrogen peroxide and peroxidase. The reagent composition of the invention is improved in storage stability and has a wider determination range. Also provided is a dry analysis element composed of reagent layer containing the inventive reagent composition.

Claims

1. A reagent composition for the quantitative analysis of inorganic phosphorus comprising xanthosine, purine nucleoside phosphorylase, xanthine oxidase, peroxidase and a color-former, said color-former being a precursor for forming a dye in the presence of hydrogen peroxide produced by said xanthine oxidase and said peroxidase.

2. The reagent composition of claim 1, further comprising a buffer composition for maintaining the aqueous solution of said reagent composition within a pH range of from 6.0 to 8.0.

3. The reagent composition of claim 1, wherein said color-former is selected from the group consisting of a leuco dye, and a composition containing a compound which forms a dye upon coupling with another compound when oxidized.

4. A dry analysis element for the quantitative analysis of inorganic phosphorus comprising a reagent layer which contains xanthosine, purine nucleoside phosphorylase, xanthine oxidase, peroxidase and a color-former, said color-former being a precursor for forming a dye in the presence of hydrogen peroxide produced by said xanthine oxidase and said peroxidase.

5. The dry analysis element of claim 4, wherein said element has a multi-layer structure comprising, in addition to said reagent layer, any one or more of a support, a spreading layer, a detection layer, a light-shielding layer, an adhesive layer, a water absorbing layer and an undercoating layer.

6. The dry analysis element of claim 4, wherein said reagent layer is partitioned into plural layers including a first reagent layer containing said xanthosine and said purine nucleoside phosphorylase, a second reagent layer containing said xanthine oxidase and a third reagent layer containing said peroxidase and said color-former.

7. The dry analysis element of claim 4, wherein said reagent layer is partitioned into two layers including a first reagent layer containing said xanthosine and said purine nucleoside phosphorylase and a second reagent layer containing said xanthine oxidase, said peroxidase and said color-former.

8. The dry analysis element of claim 4, wherein said reagent layer is partitioned into two layers including a first reagent layer containing said xanthosine, said purine nucleoside phosphorylase and xanthine oxidase, and a second reagent layer containing said peroxidase and said color-former.

9. The dry analysis element of claim 4, wherein said reagent layer comprises a water-permeable layer, a material for said water-permeable layer being selected from the group consisting of porous materials and hydrophilic polymer binders.

10. The dry analysis element of claim 4, wherein said color-former is selected from the group consisting of a leuco dye, and a composition containing a compound which forms a dye upon coupling with another compound when oxidized.