Photosensor with multiple light sources

• US 5,497,769 A
• Filed: 12/16/1993
• Issued: 03/12/1996
• Est. Priority Date: 12/16/1993
• Status: Expired due to Term

• Current Assignee: Board of Trustees of The University of Illinois, ISS
• Original Assignee: Board of Trustees of The University of Illinois, ISS
• Inventors: Fantini, Sergio, Gratton, Enrico, Barbieri, Beniamino, Mantulin, William, Franceschini, Maria A.
• Primary Examiner(s): Sykes, Angela D.
• Assistant Examiner(s): NASSER, ROBERT L
• Application Number:
  US08/168,813
• Time in Patent Office: 817 Days
• Field of Search: 128/633-634, 128/664-666, 356/35-41
• US Class Current: 600/323
• CPC Class Codes: A61B 2562/0233 Special features of optical sensors or probes classified in A61B5/00
• CPC Class Codes: A61B 2562/046 in a matrix array
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/14552 Details of sensors specially adapted therefor
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

The quantitative determination of various materials in highly scattering media such as living tissue may be determined in an external, photometric manner by the use of a plurality of light sources positioned at differing distances from a sensor. The light from said sources is amplitude modulated, and, in accordance with conventional frequency domain fluorometry or phosphorimetry techniques, the gain of the sensor is modulated at a frequency different from the frequency of the light modulation. Data may be acquired from each of the light sources at differing distances at a frequency which is the difference between the two frequencies described above. From these sets of data from each individual light source, curves may be constructed, and the slopes used to quantitatively determine the amount of certain materials present, for example oxyhemoglobin and deoxyhemoglobin in living tissue.

Claims

1. A sensor instrument for use in measuring parameters of a highly scattering medium, which comprises:

a sensor head having a face for contact with the medium;

a sensor carried on said head for detecting light striking a portion of said face;

at least one group of light sources positioned to pass said light to said face through said medium, said light sources being of differing distances from said light sensor; and

wherein all sources in each of said at least one group of sources emit light of substantially the same wavelength;

a timing circuit for causing sequential illumination and shutting off of said light sources;

a circuit for modulating an intensity of said light sources to provide amplitude modulated light signals at a first frequency;

circuitry for providing a second signal coherent with said amplitude modulated light signals, at a second frequency, to the light sensor;

circuitry for modulating the gain of, or for multiplying the output of, said light sensor by said second signal, said second frequency being different from said first frequency; and

electronics for deriving a resultant signal from said light sensor while receiving said modulated light signals, said resultant signal being at a frequency of the difference between the first and second frequencies, and electronics to compute at least two of phase shift, DC, and AC components of said light signals which pass sequentially from said light sources through the medium to said light sensor.

2. The sensor instrument of claim 1 which further comprises a shield to prevent the direct access of light from said light sources to said light sensor without passing through said medium.

3. The sensor instrument of claim 1 in which a processor is provided for computing the slopes of at least two of said phase shift, DC, and AC components provided by signals from said at least one group of light sources of differing distances.

4. The sensor instrument of claim 3 in which said processor is also for computing at least one of a scattering and an absorption coefficient of the medium being measured from said slopes.

5. The sensor instrument of claim 4 in which said medium is tissue, and said processor computes absolute concentrations of at least one of oxyhemoglobin and deoxyhemoglobin present in said tissue, making use of a computed absorption coefficient, and means for display of said concentration as a real time value.

6. The sensor of claim 5, in which said at least one group of sources includes a first group that emit light of a first wavelength that is strongly absorbed by oxyhemoglobin and a second group of sources that emit light of a second, different wavelength, that is strongly absorbed by deoxyhemoglobin.

7. The sensor instrument of claim 1 in which the intensity of light from said light sources is amplitude modulated at 10-500 MHz, and the second frequency of the second signal differs from said first frequency by about 10 Hz-100 KHz.

8. The sensor of claim 1 in which one of said groups of light sources emits light having a wavelength of about 650 nm to about 1,000 nm.

9. The sensor of claim 1 in which at least three of said light sources of differing distances are present.

10. The sensor of claim 8 in which said at least one group of sources includes a first group of sources emitting light at a first wavelength and a second group of sources emitting light light at a second, different, wavelength, said groups of light sources together having at least six of said light sources, said light sources being disposed to provide pairs of light sources which are at the same distance from said sensor, to permit gathering of data at different light wavelengths.

11. The sensor instrument of claim 1 in which said electronics are also to compute at least one of a scattering coefficient and an absorption coefficient from said at least two of phase shift, DC, and AC components.

12. A sensor instrument for use in measuring parameters of body tissue, which comprises:

a sensor head having a face for contact with the skin of a patient;

a sensor carried on said head for detecting light striking a portion of said face;

light sources positioned to pass said light to said face through said tissue, said light sources being of differing distances from said light sensor, a first group of said light sources of differing distances emitting light of one frequency and a second group of said light sources of differing distances emitting light of another frequency;

a timing circuit for causing sequential illumination and shutting off of said light sources;

a circuit for modulating an intensity of said light sources to provide amplitude modulated light signals at a first frequency;

circuitry for providing a second signal coherent with said amplitude modulated light signals, at a second frequency, to the light sensor;

circuitry for modulating the gain of, or for multiplying the output of, the light sensor by said second signal, said second frequency being different from said first frequency;

electronics for deriving a resultant signal from said light sensor while receiving said modulated light signals, said resultant signal being at a frequency of the difference between the first and second frequencies; and

a processor for receiving said resultant signal and determining at least two of phase shift, DC, and AC components of said light signals which pass sequentially from said light sources through the tissue to said light sensor, said processor also being for computing the slopes of said at least two of said phase shift, DC, and AC components provided by signals from said plurality of light sources of differing distances.

13. The sensor instrument of claim 12 in which said processor is capable of computing at least one of scattering and absorption coefficients of the tissue being measured from said slopes.

14. The sensor instrument of claim 13 in which said processor is capable of computing absolute concentrations of at least one of oxyhemoglobin and deoxyhemoglobin present in said tissue making use of said computed absorption coefficient, said sensor instrument having a display panel for displaying said concentration as a real time value.

15. The sensor instrument of claim 14, in which said first group of sources emit light of a wavelength that is strongly absorbed by oxyhemoglobin and said second group of sources emit light of a wavelength that is strongly absorbed by deoxyhemoglobin.

16. The sensor of claim 14 in which the intensity of light from said light sources is amplitude modulated at 10-500 MHz, and the second frequency of the second signal differs from said first frequency by about 10 Hz to 100 KHz.

17. The sensor instrument of claim 16 which further comprises a shield to prevent the direct access of light from said light sources to said light sensor without passing through said tissue.

18. The sensor instrument of claim 12 in which said processor is also for computing at least one of a scattering coefficient and an absorption coefficient from said slopes.

19. A sensor instrument for use in photometrically determining parameters of a highly scattering medium, which comprises:

a sensor head having a face for contact with said medium;

a sensor carried on said head for detecting light striking a portion of said face;

at least one group of light sources positioned to pass said light to said face through said medium, said light sources being of differing distances from said light sensor; and

wherein all sources in each of said at least one group of sources emit light of substantially the same wavelength;

a timing circuit for causing sequential illumination and shutting off of said light sources;

a circuit for modulating an intensity of said illuminated light sources to provide amplitude modulated light signals at a first frequency;

electronics for providing frequency domain spectroscopy electronic processing of signals received by said sensor to provide a resultant signal which is a function of the modulated light signals received by said sensor; and

a processor for receiving said resultant signal and determining at least two of phase shift, DC, and AC components of said light signals which pass sequentially from said light sources through said highly scattering media to said light sensor.

20. The sensor instrument of claim 19 in which said processor is capable of computing the slopes of at least two of said phase shift, DC, and AC components provided by light signals to said sensor from said plurality of light sources of differing distances.

21. The sensor instrument of claim 20 in which said at least one group of sources includes a first group of sources emitting light at a first wavelength and a second group of sources emitting light at a second, different, wavelength each group of said light sources emitting light at a different wavelength.

22. The sensor instrument of claim 20 in which said processor is also for determining at least one of a scattering coefficient and an absorption coefficient from said at least two of phase shift, DC, and AC components.

24. The sensor instrument of claim 22 in which said medium is tissue and said electronics computes absolute concentrations of at least one of oxyhemoglobin and deoxyhemoglobin present in said tissue making use of said computed absorption coefficients and their slopes;

and a readout display of said concentration as a real time value.

23. A sensor instrument for use in determining at least one of the scattering coefficient and absorption coefficient of a highly scattering medium, which comprises:

a sensor head having a face for contact with the medium;

a sensor carried on said head for detecting light striking a portion of said face;

at least one group of light sources positioned to pass light to said face through said medium, said light sources being of differing distances from said light sensor and wherein all sources in each of said at lest one group of sources emit light of substantially the same wavelength;

a timing circuit for causing sequential illumination and shutting off of said light sources; and

electronics to compute at least two of phase shift, DC, and AC components of light from said light sources which has passed sequentially from said light sources through the medium to said light sensor, and to compute at least one of the scattering and absorption coefficients for said light sources.

25. The sensor of claim 23 in which one of the groups of sources emits light having a wavelength from about 650 nm to about 1000 nm.

26. The sensor of claim 23, in which said at least one group of sources includes a first group that emit light of a first wavelength that is strongly absorbed by oxyhemoglobin and a second group of sources that emit light of a second, different wavelength, that is strongly absorbed by deoxyhemoglobin.

27. A sensor instrument for use in measuring parameters of a highly scattering medium, which comprises:

a sensor head having a face for contact with the medium;

a sensor carried on said head for detecting light striking a portion of said face;

at least one group of light sources positioned to pass said light to said face through said medium, said light sources being of differing distances from said light sensor, and wherein all sources in each of said at least one group of sources emit light of substantially the same wavelength;

a timing circuit for causing sequential illumination and shutting off of said light sources;

a circuit for modulating an intensity of said light sources to provide amplitude modulated light signals at a first frequency;

circuitry for providing a second signal coherent with said amplitude modulated light signals, at a second frequency, to the light sensor;

circuitry for modulating the gain of, or for multiplying the output of, said light sensor by said second signal, said second frequency being different from said first frequency; and

electronics for deriving a resultant signal from said light sensor while receiving said modulated light signals, said resultant signal being at a frequency of the difference between the first and second frequencies, and electronics to compute at least two of phase shift, DC, and AC components of said resultant signals carrying information about the medium which is sequentially traversed through by the light signals emitted by said light sources, said electronics also being for computing at least one of a scattering coefficient and an absorption coefficient from said at least two of phase shift, DC, and AC components.