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Mesoscale polynucleotide amplification device and method

  • US 5,498,392 A
  • Filed: 09/19/1994
  • Issued: 03/12/1996
  • Est. Priority Date: 05/01/1992
  • Status: Expired due to Term
First Claim
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1. A device for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising:

  • a solid substrate which is microfabricated to define;

    a sample inlet port;

    a mesoscale flow system comprising;

    a sample flow channel extending from said inlet port; and

    a polynucleotide amplification reaction chamber, in fluid communication with said flow channel;

    said sample flow channel and said reaction chamber having at least one cross-sectional dimension of width or depth which is about 0.1 to 500 μ

    m, said chamber and said channel being of dissimilar dimension; and

    a fluid exit port in fluid communication with said flow system; and

    means for thermally cycling the contents of said chamber whereby in each cycle the temperature is controlled to dehybridize double stranded polynucleotide and to permit the in vitro amplification of a preselected polynucleotide.

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