Method for producing potato microtubers
First Claim
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1. A method for producing potato microtubers, said method comprising culturing potato plants in a medium containing low sugar under illumination, followed by culture in a medium containing high sugar in the dark, wherein the improvement comprises:
- (a) culturing potato plants in a medium containing 0.5-5% w/v sugar under an illumination regimen of about 12 to about 24 hours per day for about 3 to about 6 weeks; and
(b) culturing the resulting potato plants in a medium containing 6-10% w/v sugar for about 1 to about 4 weeks under an illumination regimen of either about 6 to about 12 hours per day or about 12 to about 24 hours per day prior to culturing said plants in the dark to produce said microtubers.
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Abstract
An improved method for producing potato microtubers by tissue culture is provided. The improvement comprises culturing potato plants in a medium containing a high sugar content, i.e., 8% (w/v), for about 1 to 4 weeks under the light prior to culturing the plants in the dark to produce the microtubers.
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Citations
20 Claims
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1. A method for producing potato microtubers, said method comprising culturing potato plants in a medium containing low sugar under illumination, followed by culture in a medium containing high sugar in the dark, wherein the improvement comprises:
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(a) culturing potato plants in a medium containing 0.5-5% w/v sugar under an illumination regimen of about 12 to about 24 hours per day for about 3 to about 6 weeks; and (b) culturing the resulting potato plants in a medium containing 6-10% w/v sugar for about 1 to about 4 weeks under an illumination regimen of either about 6 to about 12 hours per day or about 12 to about 24 hours per day prior to culturing said plants in the dark to produce said microtubers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for producing potato microtubers, comprising, in the order mentioned, the steps of:
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culturing potato plants in a medium containing 0.5-5% w/v sugar under an illumination regimen of about 12 to about 24 hours per day for about 3 weeks to about 6 weeks, culturing the resulting potato plants in a medium containing 6-10% w/v sugar for about 1 to about 4 weeks under an illumination regimen of either about 6 to about 12 hours per day or about 12 to about 24 hours per day, and culturing said potato plants in the dark until microtubers are produced.
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15. A method for producing potato microtubers, said method comprising culturing potato plants in a medium containing low sugar under illumination, followed by culture in a medium containing high sugar in the dark, wherein the improvement comprises:
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culturing Russet potato plants in a medium containing 0.5-5% w/v sugar under an illumination regimen of about 12 to about 24 hours per day for about 3 to about 6 weeks; culturing the resulting potato plants in a vessel having a volume of 2-4 liters containing 0.6 to 1 liter of medium at pH 5.8, containing 6-10% w/v sugar, at an aeration rate of 0.35-0.80 liters/minute at a temperature of 20°
C. for about 1 to about 4 weeks under a dark/light cycle of 8 or 16 hours light at 5,000 lux; and
thenculturing said potato plants in the dark until microtubers are produced. - View Dependent Claims (16, 17)
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18. A method for producing potato microtubers comprising, in the order mentioned, the steps of:
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1) culturing nodal cuttings of Russet potato plants in a medium having a pH in the range from 4 to 8, containing 0.5 to 5% w/v sugar, in a vessel having a volume of 2 to 4 liters containing 0.6 to 1 liter of said medium, under an illumination regimen of about 12 to about 24 hours per day at 3,000 to 10,000 lux, at a temperature of 18°
-25°
C., at an aeration rate of 0.35 to 0.8 liters/minute, for about 3 weeks to about 6 weeks;2) culturing potato plants produced in step
1) in a medium having a pH in the range from 4 to 8, containing 6 to 10% w/v sugar, in a vessel having a volume of 2 to 4 liters containing 0.6 to 1 liter of said medium, under an illumination regimen of about 6 to about 12 hours per day or about 12 to about 24 hours per day at 3,000 to 10,000 lux, at a temperature of 18°
to 25°
C., at an aeration rate of 0.35 to 0.8 liters/minute, for about 1 week to about 4 weeks; and3) culturing potato plants produced in step
2) under the same conditions as in step
2), except that said potato plants are cultured in the dark, until microtubers are produced. - View Dependent Claims (19, 20)
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Specification