Method for diagnosing diabetes mellitus and device therefor

• US 5,500,374 A
• Filed: 08/17/1993
• Issued: 03/19/1996
• Est. Priority Date: 10/05/1992
• Status: Expired due to Term

• Current Assignee: Soichi Inoue
• Original Assignee: Soichi Inoue
• Inventors: Wenzhi, Hu
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Ludlow, Jan M.
• Application Number:
  US08/107,122
• Time in Patent Office: 945 Days
• Field of Search: 436/161, 436/173, 436/63, 422/70, 73/61.52, 73/61.53, 73/61.55, 210/656, 210/660, 210/198.2
• US Class Current: 436/161
• CPC Class Codes: B01D 15/36 involving ionic interaction
• CPC Class Codes: G01N 2030/027 Liquid chromatography
• CPC Class Codes: G01N 2030/8813 biological materials
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G01N 30/461 with serial coupling of separation columns
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

A rapid, simple and reliable method and device for diagnosing diabetes mellitus under no influence of diet. The method for diagnosing diabetes mellitus involves injecting a saliva sample from a patient suspected of having diabetes mellitus into a stationary phase in a separation column, thereafter eluting a phosphate buffer as an eluent to separate analytes contained in the saliva sample, subsequently detecting the individual separated analytes with a UV detector and electrostatic ion chromatography to produce a chromatogram, and then diagnosing diabetes mellitus based on the presence or absence of a chromato-peak of the diabetes mellitus-specific component. A zwitterionic stationary phase is employed as the stationary phase and contains a support carrier and a zwitterionic charged layer formed by coating a compound having an ammonium salt portion, a sulfonate ion portion or carboxylate ion portion on the surface of the support carrier.

Claims

1. A method for aiding in the diagnosis of diabetes mellitus comprising injecting a saliva sample from a patient suspected of having diabetes mellitus into a stationary phase in a separation column, thereafter eluting a phosphate buffer as an eluent to separate analytes contained in the saliva sample, subsequently detecting the individual separated analytes with a UV detector and producing a chromatogram, and then identifying the presence or absence of a chromato-peak A of FIG. 5, said chromato-peak A representing a diabetes mellitus-specific component, wherein said stationary phase is a zwitterionic stationary phase comprising a support carrier and a zwitterionic charged layer selected from 3-[(3-cholamidepropyl)dimethyl ammonio]-1-propanesulfonate and 3-[(3-cholamidepropyl)dimethyl ammonio]-2-hydroxy-1-propanesulfonate.