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Particle bound binding component immunoassay

  • US 5,501,949 A
  • Filed: 08/15/1994
  • Issued: 03/26/1996
  • Est. Priority Date: 12/10/1985
  • Status: Expired due to Term
First Claim
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1. A method for detection or quantitation of an analyte in a solution comprising the steps of:

  • (a) contacting the solution with insoluble particles having attached thereto a binding component specific for the analyte, so as to form a suspension comprising a first complex wherein the first complex comprises the analyte, the binding component and the insoluble particle;

    (b) applying the suspension to at least a portion of a semi-permeable membrane having interstices of dimensions relative to the insoluble particles and having a thickness such that the insoluble particles are retained throughout the thickness of the semi-permeable membrane, the portion of the semi-permeable membrane retaining the insoluble particles defining an assay zone;

    (c) contacting the semi-permeable membrane containing the assay zone with a labeling component capable of specifically binding to the first complex so as to form a second complex wherein the second complex comprises the first complex and the labeling component, the semi-permeable membrane permitting the labeling component which is not bound to the first complex to pass out of the assay zone; and

    (d) measuring the signal produced by the labeling component of the second complex as an indicator of the presence or amount of the analyte present in the solution.

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