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Miniaturized biological assembly

  • US 5,503,803 A
  • Filed: 08/09/1994
  • Issued: 04/02/1996
  • Est. Priority Date: 03/28/1988
  • Status: Expired due to Term
First Claim
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1. A miniaturized assembly for containing a sample of biological material in a fluid medium and for quantitative microscopic examination of said sample, said assembly comprisingfirst and second plates in registry with and attached to one another and being substantially parallel to one another and having facing substantially parallel planar surfaces, a patterned layer located between said planar surfaces and defining a sample chamber having upper and lower ends, the lower end of said sample chamber being defined by said second plate and closed, and the upper end of said sample chamber being open, a second chamber disposed adjacent said sample chamber and being in unimpeded communication with the open upper end of said sample chamber at all times and free of elements interposed therebetween, said patterned layer including at least one of hydrophilic and hydrophobic material at said open upper end of said sample chamber and adjacent to said second chamber to allow selective exchange of material between said sample chamber and said second chamber, wherein said second chamber has a bottom wall, the upper end of said sample chamber intersects said bottom wall to define an upper edge of the sample chamber, the bottom wall of said second chamber extends outwardly of said upper edge, and said sample chamber has a depth dimension between said first and second plates.

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