×

Positional sequencing by hybridization

  • US 5,503,980 A
  • Filed: 10/17/1994
  • Issued: 04/02/1996
  • Est. Priority Date: 11/06/1992
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for determining a nucleotide sequence of a nucleic acid target by hybridization comprising the steps of:

  • (a) creating a set of nucleic acid probes wherein each probe is 14-50 nucleotides in length and has a double stranded portion, a single stranded portion, and a variable sequence within the single stranded portion which is determinable;

    (b) hybridizing the target which is at least partly single stranded to one or more of the nucleic acid probes; and

    (c) determining the nucleotide sequence of the target which is hybridized to the single stranded portion of any probe.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×