Assay for glycated blood proteins
First Claim
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1. A method of assessing a glycated blood protein in a sample, said method comprising the steps ofa) optionally hemolysing said sample to liberate cell bound glycated protein;
- b) separating said glycated blood protein and the corresponding non-glycated blood protein from said sample using a liquid phase precipitation reagent;
c) contacting said sample before or during separation of said glycated and non-glycated proteins therefrom with a first signal forming agent capable of binding to said glycated protein with substantially higher binding affinity than for the corresponding non-glycated protein;
d) optionally, contacting said sample before or during separation of said glycated and non-glycated proteins therefrom with a second signal forming agent capable of binding to said glycated protein and to said corresponding non-glycated protein; and
e) assessing the signal forming agents which have bound to said separated proteins or which have not bound to said glycated protein or said corresponding non-glycated protein;
with the proviso that where said glycated protein comprises glycated hemoglobin said first signal forming agent is a chromophore labelled boronic acid or salt thereof having an absorption maximum at above 600 nm.
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Abstract
A method of assessin glycated blood protein in a sample which comprises separating glycated and non-glycated protein using a liquid phase precipitation reagent, contacting the sample before or during the separation with a signal forming agent capable of binding preferentially to the glycated protein, and assessing the signal forming agents.
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Citations
18 Claims
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1. A method of assessing a glycated blood protein in a sample, said method comprising the steps of
a) optionally hemolysing said sample to liberate cell bound glycated protein; -
b) separating said glycated blood protein and the corresponding non-glycated blood protein from said sample using a liquid phase precipitation reagent; c) contacting said sample before or during separation of said glycated and non-glycated proteins therefrom with a first signal forming agent capable of binding to said glycated protein with substantially higher binding affinity than for the corresponding non-glycated protein; d) optionally, contacting said sample before or during separation of said glycated and non-glycated proteins therefrom with a second signal forming agent capable of binding to said glycated protein and to said corresponding non-glycated protein; and e) assessing the signal forming agents which have bound to said separated proteins or which have not bound to said glycated protein or said corresponding non-glycated protein;
with the proviso that where said glycated protein comprises glycated hemoglobin said first signal forming agent is a chromophore labelled boronic acid or salt thereof having an absorption maximum at above 600 nm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification