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Production of chromosome region specific DNA sequences and transamination

  • US 5,506,350 A
  • Filed: 08/19/1993
  • Issued: 04/09/1996
  • Est. Priority Date: 09/20/1990
  • Status: Expired due to Term
First Claim
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1. In an improved process for transaminating a polynucleotide under aqueous liquid phase conditions, said polynucleotide containing at least one deoxycytidine nucleotide per molecule, in the presence of a bisulfite catalyst and a reactive compound comprising the formula:

  • ##STR6## wherein;

    X is a divalent radical selected from the class consisting of ##STR7## R is an alkylene radical containing 2 through 12 carbon atoms inclusive, andR1 and R2 are each independently selected from the class consisting of hydrogen and lower alkyl, the improvement which comprises carrying out the transaminating in the presence of a dissolved trihaloacetate chaotrope anion while maintaining a temperature of 20°

    to 60°

    C. and a pH of 4.5 to 7.5.

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