Nutrient medium for the bioremediation of polycyclic aromatic hydrocarbon-contaminated soil

• US 5,508,194 A
• Filed: 05/18/1995
• Issued: 04/16/1996
• Est. Priority Date: 05/24/1994
• Status: Expired due to Fees

• Current Assignee: University of Akron
• Original Assignee: University of Akron
• Inventors: Cutright, Teresa J., Lee, Sunggyu
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US08/444,161
• Time in Patent Office: 334 Days
• Field of Search: 435/253.6, 435/262, 435/262.5, 435/42, 435/252.4, 435/824, 435/863
• US Class Current: 435/253.6
• CPC Class Codes: B09C 1/10 microbiologically, biologically or by using enzymes
• CPC Class Codes: C02F 3/34 characterised by the microorganisms used
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12P 39/00 Processes involving microorganisms of different genera in the same process, simultaneously
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: Y10S 435/824 Achromobacter
• CPC Class Codes: Y10S 435/863 Mycobacterium

Abstract

A mixed bacteria culture for biodegrading polycyclic aromatic hydrocarbon contaminants includes Achromobacter sp. and Mycobacterium sp. which have been grown together and gradually acclimated to utilize polycyclic aromatic hydrocarbons as a primary food source. The mixed bacteria culture can be utilized for in situ or ex situ bioremediation of contaminated soil, or in any of various conventional bioreactors to treat contaminated liquids such as landfill leachates, groundwater or industrial effluents. The bacteria, the nutrients used to sustain growth of the bacteria, and the products of the biodegradation of the polycyclic aromatic or other hydrocarbons are all substantially harmless to the environment. The mixed bacteria can be utilized in the presence of oxygen, or hydrogen peroxide can be used alone or in combination with oxygen as an effective alternative electron acceptor. The mixed bacteria culture of Achromobacter sp. and Mycobacterium sp. provides an environmentally safe and affordable means for rapidly and effectively eliminating a variety of polycyclic aromatic hydrocarbon contaminants from the environment.

Claims

1. A nutrient system for promoting bioremediation using a bacteria culture of one or more Achromobacter sp. and Mycobacterium sp., comprising 100 parts by weight of ammonium chloride, 5 to 20 parts by weight of hydrated magnesium sulfate, 5 to 20 parts by weight of sodium chloride, and 15 to 50 parts by weight of monobasic potassium phosphate.

2. A nutrient system as set forth in claim 1, wherein said nutrients are dissolved in water.

3. A nutrient system as set forth in claim 2, wherein said monobasic potassium phosphate is from 20 to 30 parts by weight.

4. A nutrient system as set forth in claim 2, wherein said nutrient system further includes a hydrocarbon compound having from about 6 to about 20 carbon atoms.