Apparatus and method for radio frequency spectroscopy using spectral analysis

• US 5,508,203 A
• Filed: 08/06/1993
• Issued: 04/16/1996
• Est. Priority Date: 08/06/1993
• Status: Expired due to Fees

• Current Assignee: Solid State Farms Incorporated
• Original Assignee: Solid State Farms Incorporated
• Inventors: Fuller, Milton E., Cherne, Frank J.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Carpenter, Robert
• Application Number:
  US08/103,410
• Time in Patent Office: 984 Days
• Field of Search: 324/642, 324/643, 324/644, 324/645, 324/646, 128/633, 128/664, 128/665, 128/666, 128/635, 422/82.01, 422/82.02, 436/94, 436/95, 436/79, 436/108, 436/149, 436/150, 436/151, 436/806, 436/131, 73/53.01
• US Class Current: 436/149
• CPC Class Codes: A61B 5/053 Measuring electrical impedance or conductance of a portion of the body
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/7264 Classification of physiological signals or data, e.g. using neural networks, statistical classifiers, expert systems or fuzzy systems neural networks per se G06N3/00; expert systems per se G06N5/00
• CPC Class Codes: Y10S 128/925 Neural network
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/171538 Urea or blood urea nitrogen
• CPC Class Codes: Y10T 436/203332 Hydroxyl containing

Abstract

A source of high frequency electromagnetic radiation is coupled to a specimen containing a target chemical whose presence and/or concentration is to be ascertained. Preferably the source radiation includes a plurality of high frequency spectra, at least one of which encourages energy transfer with the chemical of interest. The source radiation is coupled to the specimen via a probe pair that is also used to access a return signal representing an interaction between the source signal and the specimen. The return signal is processed to yield a spectral signature correlating to the target chemical and/or its concentration. Preferably signal processing compares frequency spectra within the source signal to spectra within a sampled return signal. The sampled return signal is a signal obtained by sampling a return signal at the probe pair and by sampling the response to the source signal of a circuit that electrically approximates the specimen. The amplitude and/or phase difference between the sampled return signal and the source signal provides recognition of the spectral signature. Target chemical concentration data may be obtained from the signature and can be displayed in a number of ways. Operation of the signal processor may be optimized using a neural network. In the preferred embodiment, the specimen is a human finger that is pressed against the probe pair, and the chemical is glucose. The invention thus permits a lay user to non-invasively determine his or her glucose level.

Claims

1. A non-invasive method for determining concentration of at least one target chemical in a specimen, the method comprising the following steps:

(a) coupling to a specimen a source electromagnetic signal containing simultaneously at least first and second frequency components that are each at least 30 MHz and include harmonics that do not exceed about 1 GHz;

(b) receiving from said specimen at least two spectral components in a return signal;

(c) processing said received at least two spectral components to produce a spectral signature having frequency-identifiable spectral components therein; and

(d) determining a concentration of at least one target chemical in said specimen based upon said spectral signature.

2. The method of claim 1, wherein step (a) includes coupling a source electromagnetic signal having at least one characteristic selected from the group consisting of:

(i) a fundamental frequency of about 30 MHz, (ii) rise and fall transition times less than about 10 ns, (iii) a duty cycle of about 50%, (iv) a power level in the range of about 0 dBm to +10 dBm, (v) said signal includes at least one spectral component promoting exchange of energy in the presence of a said target chemical in said specimen, (vi) said signal is generated from a plurality of signal sources, and (vii) said plurality of frequency components are not all harmonically related.

3. The method of claim 1, wherein said specimen has at least one characteristic selected from the group consisting of (i) said specimen includes a cell membrane, (ii) said specimen is living tissue, (iii) said specimen is human tissue, and (iv) said specimen is a human finger.

4. The method of claim 1, wherein said specimen is human tissue, and steps (a) and (b) include coupling to and receiving from said specimen via a probe pair placed in contact with a portion of said specimen.

5. The method of claim 1, wherein at least one target chemical includes at least one chemical selected from the group consisting of (i) glucoses, (ii) lactose, (iii) urea, (iv) sucrose, (v) NaCl, (vi) fructose, (vii) galactose, and (viii) an alcohol.

6. The method of claim 1, wherein at least some of said plurality of frequency components are harmonically related, and wherein step (c) includes comparing at least two harmonics of said source electromagnetic signal with said at least two harmonics of said source electromagnetic signal as are present in said return signal.

7. The method of claim 1, wherein said source electromagnetic signal has a fundamental frequency of about 30 MHz and includes harmonics thereof, and wherein step (c) includes comparing N harmonics of said 30 MHz signal with said N harmonics of said 30 MHz signal as are present in said return signal, where N is an integer≧

2.

8. The method of claim 1, wherein step (c) includes passing said received at least two spectral components through a bank of filters having at least one characteristic selected from the group consisting of (i) said bank includes at least two switchably selected bandpass filters whose passband passes a chosen frequency component of said source electromagnetic signal, and (ii) said bank resolves said received at least two spectral components into a plurality of bandpass regions each region being at least 100 MHz wide.

9. The method of claim 1, wherein step (c) includes the step of forming a sampled return signal from the response of said source electromagnetic signal of a calibration circuit, and from the response of said source electromagnetic signal to said specimen.

10. The method of claim 9, wherein step (c) includes the step of comparing frequency components present in said sampled return signal with frequency components present in said source electromagnetic signal.

11. The method of claim 4, wherein step (c) includes the additional step of correcting for environmental error associated with said probe pair, said environmental error including at least one component selected from the group consisting of temperature error and pressure error of said probe pair against said specimen.

12. The method of claim 11, wherein said additional step of correcting includes comparing a fundamental frequency of said return signal with a fundamental frequency of said source electromagnetic signal.

13. The method of claim 9, wherein step (c) includes digital signal processing said sampled return signal and said source electromagnetic signal, said digital signal processing occurring under control of a microprocessor.

14. The method of claim 1, wherein step (d) includes correlating a concentration of said target chemical associated with said spectral signature with a concentration level of said target chemical known to represent a relational value, said step of correlating employing at least one element selected from the group consisting of (i) a look-up table, (ii) a neural network, and (iii) an algorithm.

15. The method of claim 1, further including the step of optimizing said processing step (c) and said determining step (d) with a neutral network.

16. The method of claim 1, further including the step of displaying information associated with said concentration of said target chemical so correlated.

17. An apparatus for non-invasively determining concentration of at least one target chemical in a specimen, comprising:

(a) means for providing a source electromagnetic signal containing simultaneously at least first and second frequency components that are each at least about 30 MHz and include harmonica that do not exceed about 1 GHz;

(b) means for coupling said source electromagnetic signal to a specimen;

(c) means for receiving from said specimen at least two spectral components in a return signal;

(d) means for processing said received at least two spectral components to produce a spectral signature having frequency-identifiable spectral components therein; and

(e) means for determining a concentration of at least one target chemical in said specimen based upon said spectral signature.

18. The apparatus of claim 17, wherein said means for providing includes a transmitter whose source electromagnetic signal has at least one characteristic selected from the group consisting of:

(i) a fundamental frequency of about 30 MHz, (ii) rise and fall transition times less than about 10 ns, (iii) a duty cycle of about 50%, (iv) a power level in the range of about 0 dBm to +10 dBm, (v) said signal includes at least one spectral component promoting exchange of energy in the presence of a said target chemical in said specimen, (vi) said signal is generated from a plurality of signal sources, and (vii) said plurality of frequency components are not all harmonically related.

19. The apparatus of claim 17, wherein said specimen has at least one characteristic selected from the group consisting of (i) said specimen includes a cell membrane, (ii) said specimen is living tissue, (iii) said specimen is human tissue, and (iv) said specimen is a human finger.

20. The apparatus of claim 17, wherein said specimen is human tissue, and said means for receiving and said means for processing include a common probe pair, placed in contact with a portion of said specimen, said common probe pair being coupled to said means for receiving and to said means for processing.

21. The apparatus of claim 17, wherein said a target chemical includes at least one chemical selected from the group consisting of (i) glucose, (ii) lactose, (iii) urea, (iv) sucrose, (v) NaCl, (vi) fructose, (vii) galactose, and (viii) an alcohol.

22. The apparatus of claim 17, wherein said plurality of frequency components includes components that are harmonically related, and wherein said means for processing compares at least two harmonics of said source electromagnetic signal with said at least two harmonics of said source electromagnetic signal as are present in said return signal.

23. The apparatus of claim 17, wherein said source electromagnetic signal has a fundamental frequency of about 30 MHz and includes harmonics thereof, and wherein said means for processing compares N harmonics of said 30 MHz signal with said N harmonics of said 30 MHz signal as are present in said return signal, where N is an integer ≧

2.

24. The apparatus of claim 17, wherein said means for processing passes said received at least two spectral components through a bank of filters having at least one characteristic selected from the group consisting of (i) said bank includes at least two switchably selected bandpass filters whose passband passes a chosen frequency component of said source electromagnetic signal;

and (ii) said bank resolves said received at least two spectral components into a plurality of bandpass regions each region being at least 100 MHz wide.

25. The apparatus of claim 17, wherein said means for processing forms a sampled return signal from the response of said source electromagnetic signal of a calibration circuit, and from the response of said source electromagnetic signal to said specimen.

26. The apparatus of claim 17, wherein said means for processing compares frequency components present in said sampled return signal with frequency components present in said source electromagnetic signal.

27. The apparatus of claim 20, further including means for correcting for environmental error associated with said probe pair, said environmental error including at least one component selected from the group consisting of temperature error and pressure error of said probe pair against said specimen.

28. The apparatus of claim 27, wherein said means for correcting compares a fundamental frequency of said return signal with a fundamental frequency of said source electromagnetic signal.

29. The apparatus of claim 25, wherein said means for processing digitally signal processes said sampled return signal and said source electromagnetic signal, said digital signal processing occurring under control of a microprocessor.

30. The apparatus of claim 17, wherein said means for correlating correlates a concentration of said target chemical associated with said spectral signature with a concentration level of said target chemical known to represent a relational value, said means for correlating employing at least one element selected from the group consisting of (i) a look-up table, (ii) a neural network, and (iii) an algorithm.

31. The apparatus of claim 17, further including neural network means for optimizing operations of said means for processing and said means for correlating.

32. The apparatus of claim 17, further including means for displaying information associated with said concentration of said target chemical so correlated.