Immunologically active conjugates and method for their preparation

• US 5,514,559 A
• Filed: 03/29/1994
• Issued: 05/07/1996
• Est. Priority Date: 03/29/1993
• Status: Expired due to Fees

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Huber, Erasmus, Hoess, Eva, Markert-Hahn, Christine, Ofenloch-Haehnle, Beatus
• Primary Examiner(s): CEPERLEY, MARY
• Application Number:
  US08/219,468
• Time in Patent Office: 770 Days
• Field of Search: 435/7.5, 435/188, 435/7.92, 530/391.9, 530/807, 530/391.5, 530/811, 436/512, 436/532
• US Class Current: 435/7.92
• CPC Class Codes: C07K 19/00 Hybrid peptides , i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
• CPC Class Codes: G01N 33/531 Production of immunochemical test materials
• CPC Class Codes: Y10S 530/807 Hapten conjugated with peptide or protein
• CPC Class Codes: Y10S 530/811 Peptides or proteins is immobilized on, or in, an inorganic carrier

Abstract

Novel conjugate of the general formula

A--L.sub.1 --L.sub.2 --B

wherein A and B are the substances to be coupled selected from the groups of haptens, proteins, polysaccharides, soluble polystyrenes, or peptides, L₁ and L₂ are trivalent linkers and L₁ and L₂ are bound via two thioether. They can be used in immunological assays and exhibit an improved storage stability.

Claims

1. A conjugate of the formula

View Dependent Claims (2, 3, 4, 10, 11, 12, 13)

2. The conjugate according to claim 1, wherein A and B are linked to L₁ and L₂, respectively, by hydroxysuccinimide or carbodiimidazolyl residues.

3. The conjugate according to claim 1, wherein at least one of A and B are selected from the group consisting of antibodies, antibody fragments, enzymes, haptens, biotin, streptavidin and peroxidase.

4. An immunological assay using a bound analyte to bind to a specific binding partner thereof, the improvement comprising the use of a conjugate according to claim 1 as the bound analyte, wherein said analyte is A or B.

10. The assay according to claim 4, wherein A is the analyte and B is a label or a support for A.

11. The conjugate according to claim 1, wherein at least one of A and B is selected from the group consisting of an antibody, an antibody fragment, an enzyme or a hapten.

12. The conjugate according to claim 1, wherein one of A and B is selected from the group consisting of biotin, streptavidin and peroxidase.

13. The conjugate according to claim 12, wherein the other of A and B is selected from the group consisting of antibodies, antibody fragments, enzymes and haptens.

5. A method for producing a conjugate of the formula

View Dependent Claims (6, 7, 8, 9)

6. The method according to claim 5, wherein A and B are linked to L₁ and L₂, respectively, via hydroxysuccinimide or carbodiimidazolyl groups.

7. The method according to claim 5, wherein at least one of A and B is selected from the group consisting of an antibody, an antibody fragment, an enzyme and a hapten.

8. The method according to claim 5, wherein one of A and B is selected from the group consisting of biotin, streptavidin and peroxidase.

9. The method according to claim 8, wherein the other of A and B is selected from the group consisting of antibodies, antibody fragments, enzymes and haptens.