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Recovery of oligonucleotides by gas phase cleavage

  • US 5,514,789 A
  • Filed: 04/21/1994
  • Issued: 05/07/1996
  • Est. Priority Date: 04/21/1994
  • Status: Expired due to Term
First Claim
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1. A method for recovering oligonucleotides from a solid support wherein said oligonucleotides are the synthetic products of cyanoethyl phosphoramidite oligonucleotide synthesis comprising the steps of:

  • (a) providing a solid support having synthesized oligonucleotides attached thereto, and(b) incubating the solid support in a sealed chamber containing gaseous cleavage/deprotection reagent selected from the group consisting of organic and inorganic amines, under conditions suitable to cleave or deprotect the synthesized oligonucleotides within 90 minutes.

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