Compact blood culture apparatus

• US 5,516,692 A
• Filed: 12/19/1994
• Issued: 05/14/1996
• Est. Priority Date: 01/29/1993
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Berndt, Klaus W.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): JASTRZAB, KRISANNE MARIE
• Application Number:
  US08/358,642
• Time in Patent Office: 512 Days
• Field of Search: 422/63, 422/82.05, 422/82.08, 422/82.09, 422/68.1, 436/45, 436/47, 436/682, 436/807-809, 435/284, 435/291, 435/312, 435/316, 435/286.7, 435/288.1, 435/288.7, 435/304.1, 435/303.3, 435/808, 356/73, 356/317-318, 356/417, 356/435-436, 356/442
• US Class Current: 435/286.7
• CPC Class Codes: B01F 29/321 of test-tubes or the like

Abstract

The present invention relates to an apparatus for detecting biological activities in a large number of blood culture vials. The vials are placed in discrete disc-like segments and rotated about an axis of a drum. In one preferred embodiment, agitation results from placing the axis of rotation perpendicular to the force of gravity. The present invention allows for individual vial identification, and for the application of more than one non-invasive microorganism detection method including fluorescence and scattered photon migration. Only a single detection station for each method is required for each segment and a plurality of stations may share common components, reducing cost while increasing accuracy. The vials may be inserted into a segment using a quick disconnect method and a keying method is disclosed to guarantee the correct orientation of a vial once it is inserted.

Claims

1. A compact blood culture apparatus comprising:

a housing;

a plurality of vials;

a drum rotatably mounted in said housing and having an axis disposed therein at an angle relative to a force of gravity, said drum being rotatable about said axis and including

means in said housing for rotating said drum about said axis;

means in said housing for detecting microorganisms within each of said plurality of vials in said drum using scattered photon migration;

means in said housing and on each vial for identifying each of said plurality of vials;

means in said drum for positioning each of said plurality of vials within a respective one of said plurality of bore-holes in an optimum orientation for identification by said identifying means; and

agitation means in said housing for activating rotation of said drum about said axis to perform agitation on said plurality of vials; and

said drum being constructed and arranged to simultaneously and continuously (i) move one of said plurality of vials through said detecting means and (ii) perform agitation on all of said plurality of vials when said drum is rotated about said axis.

2. A compact blood culture apparatus as recited in claim 1, wherein said axis is approximately perpendicular to said force of gravity.

3. A compact blood culture apparatus as recited in claim 1, wherein said drum further comprises a plurality of segments disposed about said axis, each of said segments having said bore-holes for receiving said vials.

4. A compact blood culture apparatus as recited in claim 3, wherein said drum further comprises a spacer disposed between two of said segments to separate them.

5. A compact blood culture apparatus as recited in claim 1, said vial identifier comprising:

a bar code label secured to an outer surface of each of the vials;

a laser adapted to project a beam of radiation;

optical system means in said housing for focusing said beam of radiation from said laser means on said bar code label; and

a first photodetector in said housing for collecting a radiation back-scattered from said bar code label when said beam of radiation from said laser means is focused on said bar code label by said optical system means.

6. A compact blood culture apparatus as recited in claim 5, wherein said positioning means comprises a second photodetector positioned to receive said back-scattered radiation when a portion of each of the vials crosses said beam.

7. A compact blood culture apparatus as recited in claim 1, wherein each of said bore-holes is shaped to receive a neck of one of the vials.

8. A compact blood culture apparatus as recited in claim 1 wherein said positioning means comprises:

a protrusion on an outer surface of each of said vials, said protrusion being received within an opening in said respective bore-hole in said drum.

9. A compact blood culture apparatus as recited in claim 8, wherein said protrusion comprises a bar code label having a rectangular shape.

10. A compact blood culture apparatus as recited in claim 7, wherein each of said bore-holes includes a quick disconnect for engaging and disengaging a respective one of said vials.

11. A compact blood culture apparatus as recited in claim 10, wherein said quick disconnect comprises a spring-clip with latch, said clip being secured to and extending from said drum on an outer surface of said respective one of said vials when inserted into a respective borehole and said latch removably engaging an outer bottom surface of said respective one of said vials which extends out of said respective borehole.

12. A compact blood culture apparatus as recited in claim 1, wherein said mechanism for agitation comprises:

a shaft extending along said axis and secured to said drum; and

a motor for driving said shaft to rotate said drum.

13. A compact blood culture apparatus as recited in claim 1, wherein said drum with said vials are mounted in an incubator to promote microorganism growth.

14. A compact blood culture apparatus as recited in claim 1, wherein said detecting means further comprises:

excitation light source means for emitting excitation light into each of said plurality of vials in said drum;

light divider means for dividing said excitation light into first and second components, and first component being used for monitoring said excitation light source and said second component being directed at one of said plurality of vials; and

light source monitor for receiving and monitoring said first component of said excitation light.

15. A compact blood culture apparatus as recited in claim 14,

wherein each of said plurality of vials further comprises a fluorescence chemical sensor disposed on an inner bottom surface thereof, and

wherein said detecting means further comprises:

optical lens means for focusing said second component of said excitation light upon said fluorescence chemical sensor; and

fluorescence light collector means for collecting a fluorescence light emitted from said fluorescence chemical sensor.

16. A compact blood culture apparatus as recited in claim 15, wherein said fluorescence light collector comprises:

collection fiber means for receiving a portion of said fluorescence light that reemerges from said fluorescence chemical sensor;

photomultiplier means for measuring said portion of said fluorescence light received by said collection fiber means; and

emission filter means disposed between said collection fiber means and said photomultiplier means for eliminating unwanted light from reaching said photomultiplier means.

17. A compact blood culture apparatus as recited in claim 14, wherein said gathering means comprises:

collection prism means for focusing and redirecting said photon light;

photomultiplier means for measuring said photon light from said collection prism means; and

collection fiber means disposed between said prism means and said photomultiplier means for transmitting said photon light to said photomultiplier means.

18. A compact blood culture apparatus comprising:

a housing;

a plurality of vials;

a drum rotatably mounted in said housing and having an axis disposed therein, said drum being rotatable about said axis and including a plurality of segments with a plurality of bore-holes formed within each of said segments, each of said bore-holes constructed and arranged to receive one of said plurality of vials;

means in said drum for positioning each of said plurality of vials within a respective one of said plurality of bore-holes in an optimum orientation for identification;

agitation means in said housing for activating rotation of said drum about said axis to perform agitation on said plurality of vials;

means in said housing for non-invasively detecting microorganisms within each of said plurality of vials in each of said segments in said drum using scattered photon migration;

means on each vial for identifying each of said plurality of vials including a bar code label on an outer surface of each of said plurality of vials and label reader means in said housing for reading said bar code label; and

said drum being arranged and constructed to simultaneously and continuously move (i) one of said plurality of vials through said detecting means to perform detection during rotation and (ii) perform agitation on all of said plurality of vials, when said drum is rotated about said axis.

19. A blood culture apparatus as recited in claim 18,

wherein each of said plurality of vials further comprises a fluorescence chemical sensor disposed on an inner bottom surface thereof, and

wherein said detecting means further includes:

collection fiber means for receiving a portion of a fluorescence light that reemerges from said fluorescence chemical sensor when reacting to an excitation light; and

photomultiplier means for receiving and measuring said fluorescence light.

20. A compact blood culture apparatus as recited in claim 18, wherein said detecting means further includes:

excitation light source means for emitting an excitation light;

collection prism means for focusing and redirecting said excitation light through each of said plurality of vials;

collection fiber adapted to receive a portion of said excitation light exiting from each of said plurality of vials; and

photomultiplier means for receiving and measuring said portion of said excitation light.

21. A compact blood culture apparatus as recited in claim 18, wherein said label reader means further comprises:

laser means for generating a beam of radiation;

optical system means for directing said beam on said bar code label; and

a first photodetector for collecting a radiation back-scattered from said bar code label when said beam is directed on said bar code label.

22. A compact blood culture apparatus as recited in claim 21, wherein said positioning means comprises a second photodetector positioned to receive said back-scattered radiation when a portion of one of the vials crosses said beam.

23. A compact blood culture apparatus as recited in claim 22, wherein said second photodetector is positioned to receive said back-scattered radiation when a bottom outer surface of one of the vials crosses said beam.

24. A compact blood culture apparatus as recited in claim 22, wherein said laser means, said optical system means, said first photodetector, and said second photodetector are mounted to a common plate located on the bottom of said housing under said drum.

25. A compact blood culture apparatus as recited in claim 18, wherein said positioning means further includes:

a protrusion comprising said label and having a rectangular shape; and

an opening in each of said bore-holes constructed and arranged to receive said protrusion.

26. A compact blood culture apparatus as recited in claim 18, wherein each of said bore-holes includes a quick disconnect for engaging and disengaging a respective one of said vials when inserted into a respective borehole, said quick disconnect comprising a plurality of spring-clips with an integral latch, each of said spring-clips being secured to said segments at each of said bore-holes and extending outwardly away from said segments so that said latch disengagingly engages an outer bottom surface of each of the vials which extends out of said respective borehole.

27. A compact blood culture apparatus as recited in claim 18, wherein said detecting means is secured to a baseplate located on the bottom of said housing under said drum.