Confocal tandem scanning reflected light microscope
First Claim
1. A method for aligning an adjustable confocal tandem scanning reflected light microscope having an optical system with an objective lens, a scanning disc and position and size adjustment means, said method comprising the steps of;
- (1) passing light through holes in one area of a scanning disc through part of the optical system, and then through holes in a second area of the scanning disc,(2) observing and analyzing the initial Moire patterns of light passing through said holes in said second area of the scanning disc,(3) adjusting the microscope in response to the initial Moire patterns observed in said observing step, and(4) observing the results of said adjusting step by analyzing any change in said Moire patterns as a result of said adjusting step, to determine whether alignment has been achieved.
1 Assignment
0 Petitions
Accused Products
Abstract
An improved method and apparatus for easily and accurately aligning confocal tandem scanning reflected light microscopes having scanning disc hole diameters as small as 20 microns are described. The method involves the observation and analysis of Moire and other patterns of light occurring within the microscope, and using the apparatus to make very sensitive adjustments that result in a fully aligned, rugged, and stable confocal tandem scanning reflected light microscope offering improved resolution, contrast, and optical slicing capability.
-
Citations
5 Claims
-
1. A method for aligning an adjustable confocal tandem scanning reflected light microscope having an optical system with an objective lens, a scanning disc and position and size adjustment means, said method comprising the steps of;
-
(1) passing light through holes in one area of a scanning disc through part of the optical system, and then through holes in a second area of the scanning disc, (2) observing and analyzing the initial Moire patterns of light passing through said holes in said second area of the scanning disc, (3) adjusting the microscope in response to the initial Moire patterns observed in said observing step, and (4) observing the results of said adjusting step by analyzing any change in said Moire patterns as a result of said adjusting step, to determine whether alignment has been achieved. - View Dependent Claims (2, 3, 4, 5)
-
Specification