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Immunoassays empolying generic anti-hapten antibodies and materials for use therein

  • US 5,518,887 A
  • Filed: 12/01/1993
  • Issued: 05/21/1996
  • Est. Priority Date: 03/30/1992
  • Status: Expired due to Fees
First Claim
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1. An immunoassay method for detecting the presence or measuring the amount of an analyte (A) in a test sample comprising:

  • a) exposing the test sample to a particle-analyte (P-A) and a common antibody pool to form a reaction mixture, wherein said particle-analyte (P-A) comprises a particle (P) attached to analyte (A) and said common antibody pool comprises (1) at least two antisera, one of said at least two antisera containing an antibody α

    A, the other of said at least two antisera containing an antibody α

    B, or (2) at least one antisera and at least one monoclonal antibody, said at least one antisera containing an antibody α

    A and said monoclonal antibody being antibody α

    B, or (3) at least two monoclonal antibodies, one of said at least two monoclonal antibodies being antibody α

    A, the other of said at least two monoclonal antibodies being antibody α

    B, wherein antibody α

    A and antibody α

    B have different binding specificities for analyte (A), one of which antibodies α

    A or α

    B is also able to cross-react with other substances that are structurally related to analyte (A), while the other antibody cannot cross-react with said substances;

    b) allowing the formation of complexes including (P-A)(α

    A), or (P-A)(α

    B), or (P-A)(α

    B)(α

    A), or combinations of the three; and

    c) detecting the presence or measuring the amount of any of (P-A)(α

    A), or (P-A)(α

    B), or (P-A)(α

    B)(α

    A), or combinations of the three in the absence of analyte (A);

    or the absence of (P-A)(α

    A), or (P-A)(α

    B), and (P-A)(α

    B)(α

    A) in the presence of analyte (A).

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