Compact blood culture apparatus

• US 5,518,923 A
• Filed: 06/06/1995
• Issued: 05/21/1996
• Est. Priority Date: 06/06/1995
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Berndt, Klaus W., Schwarz, Daniel L.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US08/470,288
• Time in Patent Office: 350 Days
• Field of Search: 422/64, 422/82.08, 435/286, 435/291, 435/312, 435/801, 435/808, 435/809, 356/39, 356/73, 356/319, 356/417, 356/440
• US Class Current: 435/287.3
• CPC Class Codes: C12M 23/08 Flask, bottle or test tube
• CPC Class Codes: C12M 27/10 Rotating vessel
• CPC Class Codes: C12M 41/14 Incubators; Climatic chambers per se B01L1/00
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: G01N 2035/00306 Housings, cabinets, control panels (details)
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0458 Multiple concentric rows of wells
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: Y10S 435/801 Anerobic cultivation
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers

Abstract

The present invention describes an apparatus for detecting biological activities in a large number of blood culture containers. The containers are placed in a plurality of wells arranged in concentric rows on a turntable that is rotated about a central axis. Each well is designed to receive and hold one of a plurality of sealable containers inserted base first, with each container having optical sensing means for sensing microorganisms therein and a bar code pattern attached thereto for identification purposes. Each well has an opening in its base to allow a sensor station to monitor a fluorescence chemical sensor in each container to determine whether there is microorganism growth within the container. Prior to inserting each container into a well on the turntable, a culture medium and blood specimen are introduced into the container and the bar code is scanned to identify the container. As the containers are rotated on the turntable they are exposed to conditions enabling a variety of metabolic, physical, and chemical changes to take place in the presence of microorganisms within the specimen. The apparatus also includes a door on the front that opens and permits the turntable to be loaded and unloaded from the front of the apparatus with simultaneous access to all containers.

Claims

1. A compact blood culture apparatus comprising:

an incubator providing an environment for culturing,

a turntable rotatable about an axis having a plurality of wells in a top surface for receiving a plurality of containers;

a mechanism for rotating said turntable about said axis;

at least one sensor station for detecting microorganism growth within each container as said turntable is rotated about said axis; and

wherein each of said plurality of wells includes an opening through the bottom of said turntable and said turntable is positioned and arranged to provide visable access to said container by said at least one sensing station through said opening.

2. A compact blood culture apparatus according to claim 1, wherein said turntable includes a plurality of concentric rows of said plurality of wells, wherein each of said concentric rows corresponds to said at least one sensor station for detecting microorganism growth within said plurality of containers contained in said one row as each of said plurality of containers in said one row passes over said at least one sensor station.

3. A compact blood culture apparatus according to claim 1, wherein each of said plurality of conatiners includes a base and a neck and said base of each of said plurality of containers is received in one of said wells in said top surface of said turntable.

4. A compact blood culture apparatus according to claim 1, wherein said turntable is rotated by a motor attached to the periphery of said turntable.

5. A compact blood culture apparatus according to claim 1, wherein one of said plurality of wells contains a calibration container for calibrating said sensor station.

6. A compact blood culture apparatus according to claim 1, wherein said turntable is mounted on said axis using a quick disconnect that allows said turntable to be removed from said apparatus and replaced with another turntable.

7. A compact blood culture apparatus according to claim 1, wherein rotation of said turntable is periodically stopped by said mechanism so that each container is positioned at at least one sensor station for detecting microorganism growth within said container.

8. A compact blood culture apparatus according to claim 1, wherein each container passes by at least one sensor station for detecting microorganism growth within said container as said turntable is rotated about said axis by said mechanism.