Diagnostic flow cell device
First Claim
1. A multi flat-layered diagnostic flow cell for determining the presence or amount of an analyte in a test sample, said flow cell being reusable for a plurality of test samples, and comprising:
- i) a hydrophobic spacing layer disposed between a first and a second opposed substrate, said first and second substrates being chemically inert and non-conductive, and wherein said spacing layer has a longitudinal void and wherein said spacing layer and said opposed substrates define a flow channel;
ii) fastening means for coupling said spacing layer and said opposed substrates;
iii) inlet means for permitting said sample to enter said flow channel;
iv) outlet means for permitting said sample to exit said flow channel; and
v) means for producing a detectable signal, wherein said means is at least partially contained within said flow channel;
said means comprising a working electrode containing an immobilized bioreagent enzyme dispersed within a latex resin binding reagent.
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Abstract
The present invention provides a diagnostic flow cell for determining the presence or amount of an analyte which may be contained in a test sample. The flow cell comprises a spacing layer having a longitudinal void disposed between a pair of opposed substrates. The spacing layer and the opposed substrates define a flow channel wherein reagent means can be immobilized. When the immobilized reagent means is contacted with an analyte, the reagent means can produce an electrically, optically, or electrically and optically detectable response to the analyte. Hence, the reagent means that is immobilized within the flow channel can comprise (i) a counter electrode, a reference electrode and a working electrode, (ii) an optically sensitive dye or (iii) a counter electrode, a reference electrode and a working electrode and an optically sensitive dye. The flow cell can be interfaced with means for introducing a test sample into and out of the flow cell'"'"'s flow channel and detection means for detecting a signal generated by the immobilized reagent means.
The present invention also provides methods for detecting the presence or amount of an analyte which may be contained in a test sample.
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Citations
39 Claims
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1. A multi flat-layered diagnostic flow cell for determining the presence or amount of an analyte in a test sample, said flow cell being reusable for a plurality of test samples, and comprising:
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i) a hydrophobic spacing layer disposed between a first and a second opposed substrate, said first and second substrates being chemically inert and non-conductive, and wherein said spacing layer has a longitudinal void and wherein said spacing layer and said opposed substrates define a flow channel; ii) fastening means for coupling said spacing layer and said opposed substrates; iii) inlet means for permitting said sample to enter said flow channel; iv) outlet means for permitting said sample to exit said flow channel; and v) means for producing a detectable signal, wherein said means is at least partially contained within said flow channel;
said means comprising a working electrode containing an immobilized bioreagent enzyme dispersed within a latex resin binding reagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A multiflat-layered diagnostic flow cell for determining the presence or amount of one or more analytes in a test sample, said flow cell being reusable for a plurality of test samples, and comprising:
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i) a hydrophobic spacing layer disposed between a first and second opposed substrate, said first and second substrates being chemically inert and non-conductive, and wherein said spacing layer has a longitudinal void and wherein said spacing layer and said opposed substrates define a flow channel; ii) fastening means for coupling said spacing layer and said opposed substrates; iii) inlet means for permitting said sample to enter said flow channel; iv) outlet means for permitting said sample to exit said flow channel; and v) means for producing a detectable signal, wherein said means is at least partially contained within said flow channel;
said means comprising a working electrode containing at least one immobilized bioreagent dispersed within a latex resin binding reagent. - View Dependent Claims (39)
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Specification