Detection of specific sequences in nucleic acids
DCFirst Claim
1. A method of testing for the presence or absence of a target sequence in a mixture of single-stranded nucleic acid fragments, comprising:
- reacting a mixture of single-stranded nucleic acid fragments with a first probe which is complementary to a first region of the target sequence, and with a second probe which is complementary to a second region of the target sequence, where the first and second target regions are contiguous with one another, under hybridization conditions in which the two probes become stably hybridized to their associated target regions,ligating first and second probes which have hybridized to said contiguous first and second target regions, andtesting for the presence of ligated first and second probes, where the presence of the ligation product of said first and second probes indicates the presence of the target sequence in said mixture.
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Abstract
A method of testing for the presence or absence of a target sequence in a mixture of single-stranded nucleic acid fragments is disclosed. The method involves reacting a mixture of single-stranded nucleic acid fragments with a first probe which is complementary to a first region of the target sequence, and with a second probe which is complementary to a second region of the target sequence, where the first and second target regions are contiguous with one another, under hybridization conditions in which the two probes become stably hybridized to their associated target regions. Following hybridization, any of the first and second probes hybridized to contiguous first and second target regions are ligated, and the sample is tested for the presence of expected probe ligation product. The presence of ligated product indicates that the target sequence is present in the sample.
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Citations
7 Claims
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1. A method of testing for the presence or absence of a target sequence in a mixture of single-stranded nucleic acid fragments, comprising:
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reacting a mixture of single-stranded nucleic acid fragments with a first probe which is complementary to a first region of the target sequence, and with a second probe which is complementary to a second region of the target sequence, where the first and second target regions are contiguous with one another, under hybridization conditions in which the two probes become stably hybridized to their associated target regions, ligating first and second probes which have hybridized to said contiguous first and second target regions, and testing for the presence of ligated first and second probes, where the presence of the ligation product of said first and second probes indicates the presence of the target sequence in said mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification