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Monitoring bioelectrochemical reactions and novel media for bioelectrochemical reactions

  • US 5,521,101 A
  • Filed: 08/26/1993
  • Issued: 05/28/1996
  • Est. Priority Date: 08/28/1992
  • Status: Expired due to Fees
First Claim
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1. A method for determining a gaseous or vapor analyte comprising(1) providing a substantially liquid-free electrochemical cell comprising microvoltammetric electrodes including a microvoltammetric sensing electrode;

  • solid or semi-solid support consisting essentially of an electrolyte in electrical contact with the microvoltammetric sensing electrode; and

    an enzyme or synzyme;

    whereto the enzyme or synzyme is immobilized within the support in electrical contact with the microvoltammetric sensing electrode and wherein the enzyme or synzyme is contactable with a gaseous or vapor phase, and(2) exposing the electrochemical cell to a gaseous or vapor phase suspected of containing the gaseous or vapor analyte which is a substrate for the enzyme or synzyme, whereby the gaseous or vapor analyte contacts the enzyme or synzyme and reacts thereby generating a detectable electrical response relatable to presence of the analyte; and

    (3) determining the electrical response by the microvoltammetric sensing electrode to determine the presence or amount of the analyte.

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