Reusable biocompatible interface for immobilization of materials on a solid support

• US 5,521,702 A
• Filed: 06/14/1994
• Issued: 05/28/1996
• Est. Priority Date: 06/14/1994
• Status: Expired due to Term

• Current Assignee: Arizona Board of Regents (University of Arizona)
• Original Assignee: Arizona Board of Regents (University of Arizona)
• Inventors: Schmidt, Richard A., Tollin, Gordon, Salamon, Zdzislaw, Macleod, H. Angus
• Primary Examiner(s): McGraw, Vincent P.
• Assistant Examiner(s): HANTIS, KAREN
• Application Number:
  US08/259,388
• Time in Patent Office: 714 Days
• Field of Search: 356/244, 356/136, 356/445, 356/300, 356/318, 436/172, 436/165, 422/82.11, 422/82.05, 422/82.09, 435/287.1, 435/287.2, 435/287.9, 435/808, 428/66.6, 428/137-138
• US Class Current: 356/244
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658

Abstract

A method for the formation of a biocompatible film composed of a self-assembled bilayer membrane deposited on a planar surface. This bilayer membrane is capable of immobilizing materials to be analyzed in an environment very similar to their native state. Materials so immobilized may be subject to any of a number of analytical techniques.

Claims

1. A reusable biocompatible interface for immobilization of materials on a solid support, comprising:

(a) first, a self-assembled bilayer membrane wherein the exterior is hydrophobic and the interior is hydrophobic in nature;

(b) second, a holder with a shaped aperture, where said aperture is shaped to allow the formation and maintenance of the self-assembled bilayer membrane;

(c) third, a solid support on which rests the holder with the shaped aperture and the self-assembled bilayer membrane contained within the shaped aperture.

2. The holder with a shaped aperture described in claim 1, wherein said holder is made of a hydrophobic material.

3. The holder with a shaped aperture described in claim 2, wherein said holder is composed of a hydrocarbon polymer.

4. The solid support described in claim 1, wherein said support is made of glass, plastic or quartz.

5. The solid support described in claim 4, wherein said support has a thin film deposited on it.

6. The solid support described in claim 5, wherein the thin film or films are composed of metal or a semi-conducting material with a hydrophilic surface.

7. The reusable biocompatible interface described in claim 1 further comprising:

(a) first, an energy source;

(b) second, a means to shape and direct the emitted energy;

(c) third, a prism;

(d) fourth, a detector for sensing the intensity of the reflected energy passing from the energy source, in one side of the prism, through the sample area, out the opposite side of the prism and onto the detector;

(e) fifth, a rotating table upon which the prism, the sample and the detector rest.

8. The reusable biocompatible interface described in claim 7, wherein the energy source is a low power laser and the detector senses light intensity.

9. The reusable biocompatible interface described in claim 7, wherein the detector array senses electrochemical changes.