Calcium phosphate hydroxyapatite precursor and methods for making and using the same

• US 5,522,893 A
• Filed: 03/12/1993
• Issued: 06/04/1996
• Est. Priority Date: 03/12/1993
• Status: Expired due to Term

• Current Assignee: ADA Foundation
• Original Assignee: American Dental Association Health Foundation
• Inventors: Takagi, Shozo, Chow, Laurence C.
• Primary Examiner(s): LANGEL, WAYNE A
• Application Number:
  US08/030,709
• Time in Patent Office: 1,180 Days
• Field of Search: 423/305, 423/311, 423/308, 623/16, 623/11, 433/199.1, 433/201.1, 433/228.1, 106/35, 424/57, 424/440, 424/602
• US Class Current: 423/305
• CPC Class Codes: A61F 2310/00293 containing a phosphorus-containing compound, e.g. apatite
• CPC Class Codes: A61K 6/54 Filling; Sealing
• CPC Class Codes: A61K 6/838 Phosphorus compounds, e.g. apatite
• CPC Class Codes: A61K 6/864 Phosphate cements apatite A61K6/838
• CPC Class Codes: A61K 6/876 Calcium oxide
• CPC Class Codes: A61K 8/19 containing inorganic ingredients
• CPC Class Codes: A61K 8/24 Phosphorous; Compounds thereof
• CPC Class Codes: A61L 24/02 containing inorganic materials
• CPC Class Codes: A61L 27/12 Phosphorus-containing materials, e.g. apatite
• CPC Class Codes: A61Q 11/00 Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses

Abstract

Calcium phosphate compositions that are particularly useful and unique as orthopedic and dental cements and remineralizers, as well as methods and kits for their use, and the resulting products are disclosed. The compositions comprise tetracalcium phosphate which has been prepared from a mixture with a calcium to phosphorous ratio of less than 2, or prepared and maintained under substantially anhydrous conditions prior to use or, preferably, both. The novel compositions are converted substantially to hydroxyapatite upon setting, are substantially gradually resorbable and replaced by bone when implanted in contact with living bony tissue, and offer distinct advantages in terms of cement strength, setting time and reliability and other properties.

Claims

1. A calcium phosphate-containing composition, which self-hardens substantially to hydroxyapatite at ambient temperature when in contact with an aqueous medium, comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound, wherein the tetracalcium phosphate is prepared from a starting mixture of one or more sources of calcium, phosphorous and oxygen which mixture has a calcium to phosphorous ratio of less than 2.

2. A calcium phosphate-containing composition as in claim 1 wherein the other sparingly soluble calcium phosphate is selected from the group consisting of monocalcium phosphate, dicalcium phosphate anhydrous, dicalcium phosphate dihydrate, alpha-tricalcium phosphate, beta-tricalcium phosphate, octacalcium phosphate, and amorphous calcium phosphate.

3. A calcium phosphate-containing composition as in claim 2, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

17. A dental restorative having as a calcium phosphate component the composition of claim 1.

18. A remineralizing dentifrice comprising as a calcium phosphate abrasive the composition of claim 1.

19. A remineralizing chewing gum comprising as a calcium phosphate additive the composition of claim 1.

20. The dental restorative of claim 17, wherein the aqueous medium is saliva.

25. A dental desensitization agent having as a calcium phosphate component the composition of claim 1.

27. A calcium phosphate tissue substitute precursor having as a calcium phosphate component the composition of claim 1.

40. A biological implant comprised of the tissue substitute precursor of claim 27 inserted into contact with living tissue.

42. A method of bonding living tissue comprising inserting the tissue substitute precursor of claim 27 as a bridging agent between the tissues to be bonded.

4. A calcium phosphate-containing composition, which self-hardens substantially to hydroxyapatite at ambient temperature when in contact with an aqueous medium, comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound, wherein the tetracalcium phosphate is prepared and maintained under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compound.

5. A calcium phosphate-containing composition as in claim 4 wherein the sparingly soluble calcium phosphate is selected from the group consisting of monocalcium phosphate, dicalcium phosphate anhydrous, dicalcium phosphate dihydrate, alpha-tricalcium phosphate, beta-tricalcium phosphate, octacalcium phosphate, and amorphous calcium phosphate.

6. A calcium phosphate-containing composition as in claim 5, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

21. A dental restorative having as a calcium phosphate component the composition of claim 4.

22. A remineralizing dentifrice comprising as a calcium phosphate abrasive the composition of claim 4.

23. A remineralizing chewing gum comprising as a calcium phosphate additive the composition of claim 4.

24. The dental restorative of claim 21 wherein the aqueous medium is saliva.

26. A dental desensitization agent having as a calcium phosphate component the composition of claim 4.

28. A calcium phosphate tissue substitute precursor having as a calcium phosphate component the composition of claim 4.

41. A biological implant comprised of the tissue substitute precursor of claim 28 inserted into contact with living tissue.

43. A method of bonding living tissue comprising inserting the tissue substitute precursor of claim 28 as a bridging agent between the tissues to be bonded.

7. A calcium phosphate-containing composition, which self-hardens substantially to hydroxyapatite at ambient temperature when in contact with an aqueous medium, comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound, wherein the tetracalcium phosphate is free of calcium oxide.

8. A calcium phosphate-containing composition as in claim 7, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

9. A calcium phosphate-containing composition, which self-hardens substantially to hydroxyapatite at ambient temperature when in contact with an aqueous medium, comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound, wherein the tetracalcium phosphate is free of surface hydroxyapatite prior to use.

10. A calcium phosphate-containing composition as in claim 9, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

11. A calcium phosphate-containing composition, which self-hardens substantially to hydroxyapatite at ambient temperature when in contact with an aqueous medium, comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound, wherein the tetracalcium phosphate is prepared from a starting mixture of one or more sources of calcium, phosphorous and oxygen which has a calcium to phosphorous ratio of less than 2;

and wherein the tetracalcium phosphate is prepared and maintained under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compound.

12. A calcium phosphate-containing composition as in claim 11, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

13. The composition of claim 11 further comprising up to approximately 10% by weight of additional calcium or phosphate-containing compounds in an amount sufficient to alter the pH of the composition.

14. The composition of claim 11 further comprising an additive selected from the group consisting of proteins, medicaments, filler materials, crystal growth adjusters, viscosity modifiers and pore forming agents.

15. A calcium phosphate-containing composition, which self-hardens substantially to hydroxyapatite at ambient temperature when in contact with an aqueous medium, comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound, wherein the tetracalcium phosphate is comminuted tetracalcium phosphate having an average particle size for at least about 10% of the particles of about 15μ

or less and which is free of both impurity calcium oxide and surface hydroxyapatite.

16. A calcium phosphate-containing composition as in claim 15, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

29. A calcium phosphate bone cement which self-hardens substantially at ambient temperature and is substantially resorbable when implanted in contact with living bone comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate wherein the tetracalcium phosphate is prepared from a starting mixture of one or more sources of calcium, phosphorous and oxygen which has a calcium to phosphorous ratio of less than 2.

30. The cement of claim 29, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate dihydrate and dicalcium phosphate anhydrous.

31. A calcium phosphate bone cement which self-hardens substantially at ambient temperature and is substantially resorbable when implanted in contact with living bone comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate wherein the tetracalcium phosphate is prepared and maintained under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compound.

32. The cement of claim 31, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate dihydrate and dicalcium phosphate anhydrous.

38. A biological implant comprised of the cement of claim 31 inserted into contact with living tissue.

44. A method of augmenting bony tissue comprising inserting in contact with that tissue the cement of claim 31.

46. A method of repairing bony defects comprising filling the defect with the cement of claim 31.

33. A calcium phosphate bone cement which self-hardens substantially at ambient temperature and is substantially resorbable when implanted in contact with living bone comprising tetracalcium phosphate and at least one other sparingly soluble calcium phosphate wherein the tetracalcium phosphate is prepared from a starting mixture of one or more sources of calcium, phosphorous and oxygen which has a calcium to phosphorous ratio of less than 2;

and wherein the tetracalcium phosphate is prepared and maintained under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compound.

34. The cement of claim 33, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate dihydrate and dicalcium phosphate anhydrous.

35. The cement of claim 33, wherein the cement converts in major part to hydroxyapatite having a diametral tensile strength of at least 10 MPa, and is substantially resorbable and replaced by bony tissue when implanted in contact with living bone.

36. The cement of claim 35 wherein the cement sets reliably and reproducibly in less than about 30 minutes.

37. The calcium phosphate cement of claim 33 wherein the tetracalcium phosphate to dicalcium phosphate ratio is between about 1:

1 and about 1;

4.

39. A biological implant comprised of the cement of claim 33 inserted into contact with living tissue.

45. A method of augmenting bony tissue comprising inserting in contact with that tissue the cement of claim 33.

47. A method of repairing bony defects comprising filling the defect with the cement of claim 33.

48. An improved method for preparing a tetracalcium phosphate based calcium phosphate-containing composition comprising combining tetracalcium phosphate and at least one other sparingly soluble calcium phosphate, wherein the improvement comprises employing tetracalcium phosphate prepared from a mixture of one or more sources of calcium, phosphorus and oxygen which has a calcium to phosphorous ratio of less than 2.

49. The method of claim 48 wherein the other sparingly soluble calcium phosphate is selected from the group consisting of monocalcium phosphate, dicalcium phosphate anhydrous, dicalcium phosphate dihydrate, alpha-tricalcium phosphate, beta-tricalcium phosphate, octacalcium phosphate, and amorphous calcium phosphate.

50. The method of claim 49, wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

51. An improved method for preparing a tetracalcium phosphate based calcium phosphate-containing composition comprising combining tetracalcium phosphate and at least one other sparingly soluble calcium phosphate wherein the improvement comprises preparing and maintaining the tetracalcium phosphate under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphates.

52. A method as in claim 51 wherein the sparingly soluble calcium phosphate is selected from the group consisting of monocalcium phosphate, dicalcium phosphate anhydrous, dicalcium phosphate dihydrate, alpha-tricalcium phosphate, beta-tricalcium phosphate, octacalcium phosphate, and amorphous calcium phosphate.

53. A method as in claim 52 wherein the other sparingly soluble calcium phosphate is selected from the group consisting of dicalcium phosphate anhydrous and dicalcium phosphate dihydrate.

54. An improved method for preparing a tetracalcium phosphate based calcium phosphate-containing composition comprising combining tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound wherein the improvement comprises employing tetracalcium phosphate prepared from a mixture of one or more sources of calcium, phosphorous and oxygen which has a calcium to phosphorous ratio of less than 2;

and wherein the tetracalcium phosphate is prepared and maintained under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compounds.

56. A formulation for increasing the remineralization potential of saliva comprising a tetracalcium phosphate based calcium phosphate-containing composition prepared according to the method of claim 54.

55. An improved method for preparing a calcium phosphate cement which self-hardens substantially at ambient temperature comprising combining tetracalcium phosphate and at least one other sparingly soluble calcium phosphate compound wherein the improvement comprises employing tetracalcium phosphate prepared from a starting mixture of one or more sources of calcium, phosphorous and oxygen which has a calcium to phosphorous ratio of less than 2;

and preparing and maintaining the tetracalcium phosphate under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compound.

57. A self-hardenable industrial cement prepared according to the method of claim 55.

58. A paste comprising an aqueous mixture of tetracalcium phosphate and at least one other sparingly soluble calcium phosphate which aqueous mixture hardens into a cement at an ambient temperature wherein the tetracalcium phosphate is prepared from a starting mixture of one or more sources of calcium, phosphorous and oxygen which has a calcium to phosphorous ratio of less than 2.

59. A paste comprising an aqueous mixture of tetracalcium phosphate and at least one other sparingly soluble calcium phosphate which aqueous mixture hardens into a cement at an ambient temperature wherein the tetracalcium phosphate is prepared and maintained under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compounds.

60. A slurry comprising an aqueous mixture of tetracalcium phosphate and at least one other sparingly soluble calcium phosphate, both calcium phosphates being present in the slurry in excess such that hydroxyapatite is substantially continuously precipitated from the slurry at ambient temperature, wherein the tetracalcium phosphate is prepared from a starting mixture of one or more sources of calcium, phosphorous and oxygen which has a calcium to phosphorous ratio of less than 2.

61. A slurry comprising an aqueous mixture of tetracalcium phosphate and at least one other sparingly soluble calcium phosphate, both calcium phosphates being present in the slurry in excess such that hydroxyapatite is substantially continuously precipitated from the slurry at an ambient temperature, wherein the tetracalcium phosphate is prepared and maintained under substantially anhydrous conditions prior to its contact with the aqueous medium in combination with the other sparingly soluble calcium phosphate compounds.

62. The slurry of claim 61 further comprising up to approximately 10% by weight of additional calcium or phosphate containing compounds in an amount sufficient to alter the pH of the slurry.