Wash detection method for dried chemistry test elements

• US 5,525,514 A
• Filed: 04/03/1995
• Issued: 06/11/1996
• Est. Priority Date: 04/06/1994
• Status: Expired due to Fees

• Current Assignee: Johnson & Johnson Clinical Diagnostics Inc. (Johnson & Johnson)
• Original Assignee: Johnson & Johnson Clinical Diagnostics Inc. (Johnson & Johnson)
• Inventors: Mulqueen, Paul J., Jacobs, Merrit N., Marvin, Russel H.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Le, Long V.
• Application Number:
  US08/415,262
• Time in Patent Office: 435 Days
• Field of Search: 436/43, 436/46, 436/49, 436/50, 436/54, 436/55, 436/164, 436/166, 436/174, 436/805, 436/44, 436/177, 422/63, 422/64, 422/65, 422/66, 422/67, 422/105, 422/119, 422/82.05
• US Class Current: 436/46
• CPC Class Codes: G01N 2035/00138 Slides
• CPC Class Codes: G01N 2035/1018 Detecting inhomogeneities, e.g. foam, bubbles, clots
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/12 Condition responsive control
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A method for verifying the effectiveness of a washing operation on a clinical dry chemistry slide element in which wash liquid is dispensed onto the surface of the slide element and then detection devices are used to verify the effectiveness of the wash by making an initial detection at an on center site followed by making a second detection at a second and off-center radial position. A comparison of the two readings provides verification of the wash. In addition, rate measurements can be made at each of the read positions to measure the effectiveness of the wash and to verify sample application for immunological assays.

Claims

1. A method for verifying a proper washing sequence on a plurality of test elements having a chemistry portion of at least one reagent layer disposed thereon, the method comprising the steps of:

a) adding a wash liquid to each of said test elements at a first wash position located within said chemistry portion;

b) reading each of said test elements at a first read location located within said chemistry portion to detect a wash-influenced result;

c) reading each of said test elements at a second read location within said chemistry portion that is different from said first read location, to detect a wash-influenced result, one of said first and second read locations being within said first wash position;

d) comparing the readings of steps b) and c); and

e) discarding the results of the test if said reading of step b) is higher than said reading of step c) beyond normal noise variability.

2. A method as claimed in claim 1 in which step b) further comprises the step of reading said element at substantially a center of said wash position and step c) comprises reading said element at a location removed from said center of said wash position.

3. A method as claimed in claim 2 further comprising the step of applying a wash-activable coating at said removed location prior to said washing step a), wherein said coating produces a detectable signal when wetted.

4. A method as claimed in claim 1, wherein step a) comprises the step of applying wash liquid at a position located removed from the center of said chemistry portion.

5. A method as claimed in claim 1, wherein said step a) comprises adding wash liquid at an off-center location along said chemistry portion, step b) comprises reading said test element at the center of said chemistry portion and step c) comprises reading said test element at a location which is radially opposite said wash position, relative to said center of said chemistry portion.

6. A method as defined in claim 1, wherein said step a) comprises adding two wash liquids, one after the other, to either the same or a different respective location on the test element, the first of said wash liquids comprising water without any activating solution.