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Immobilized molecular synthesis of systematically substituted compounds

  • US 5,527,681 A
  • Filed: 11/05/1992
  • Issued: 06/18/1996
  • Est. Priority Date: 06/07/1989
  • Status: Expired due to Term
First Claim
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1. A method of synthesizing an array of oligonucleotides on a surface of a substrate, the method comprising the steps of:

  • identifying a target oligonucleotide having a nucleic acid monomer sequence complementary to a receptor oligonucleotide of interest;

    synthesizing an array of oligonucleotides on said surface of said substrate by the steps of activating predefined regions of said substrate and covalently coupling selected nucleic acid monomers to said substrate to form an array of oligonucleotides, said array of oligonucleotides comprising more than one group of oligonucleotides, wherein each of said more than one group consists of a sequence of nucleic acid monomers that is the same as the nucleic acid monomer sequence of said target oligonucleotide, except that each of said more than one group has a different single position in said nucleic acid monomer sequence of said target oligonucleotide substituted with each member of a basis set of nucleic acid monomers; and

    determining which oligonucleotide sequence in each of said groups of oligonucleotides specifically binds to said receptor oligonucleotide of interest.

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