Anti-CD3 antibody-aminodextran conjugates for induction of T-cell activation and proliferation

• US 5,527,713 A
• Filed: 07/19/1995
• Issued: 06/18/1996
• Est. Priority Date: 06/11/1993
• Status: Expired due to Term

• Current Assignee: Coulter International Corp. (Danaher Corporation)
• Original Assignee: Coulter Corporation (Danaher Corporation)
• Inventors: Kenyon, Norma S., Maples, John A., Healy, Cynthia G., Siiman, Olavi, Bolton, Wade E.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): CHIN, CHRISTOPHER L
• Application Number:
  US08/504,327
• Time in Patent Office: 335 Days
• Field of Search: 436/528, 436/529, 436/547, 436/548, 530/388.1, 530/391.1, 530/391.5
• US Class Current: 436/529
• CPC Class Codes: A61K 47/6849 the antibody targeting a receptor, a cell surface antigen or a cell surface determinant
• CPC Class Codes: A61K 47/6883 Polymer-drug antibody conjugates, e.g. mitomycin-dextran-Ab; DNA-polylysine-antibody complex or conjugate used for therapy
• CPC Class Codes: G01N 33/531 Production of immunochemical test materials
• CPC Class Codes: G01N 33/56972 White blood cells
• CPC Class Codes: G01N 33/56988 HIV or HTLV

Abstract

The invention describes the use of novel aminodextran compounds containing about 5-20% by weight amine groups to bind a plurality of monoclonal antibodies. The resulting antibody-aminodextran compounds may be used to induce the activation and proliferation of selected mammalian cells. Specific examples are given using an anti-CD3 monoclonal antibody conjugated two aminodextrans containing about 5% and 16%, respectively, by weight amine groups as an agent for inducing the activation and proliferation of T cells.

Claims

1. An antibody-aminodextran conjugate for induction of T-cell activation and proliferation, consisting essentially of T-cell specific antibodies covalently bound to amine groups present on a water soluble aminodextran having about 7-20% by weight amine groups and a molecular weight of at least 100,000 daltons, wherein said antibodies are of a single type each specific for the same T-cell antigen and wherein the molar ratio of said antibodies to said aminodextran is greater than or equal to two.

2. The conjugate according to claim 1 wherein said aminodextran has about 15-17% by weight amine groups.

3. The conjugate according to claim 1 wherein said antibody is a monoclonal antibody.

4. The conjugate according to claim 3 wherein said antibody is an anti-CD3 monoclonal antibody.

5. An antibody-aminodextran conjugate for induction of T-cell activation and proliferation, consisting essentially of T-cell specific monoclonal antibodies and a water soluble aminodextran having a molecular weight of at least 100,000 daltons and about 7-20% by weight amine groups to which said antibodies are conjugated, wherein said antibodies are of a single type each specific for the same T-cell antigen and wherein the molar ratio of said antibodies to said aminodextran is greater than or equal to two.

6. The conjugate according to claim 5 wherein said antibody and aminodextran are covalently linked by a bridging group.

7. The conjugate according to claim 5 wherein said aminodextran contains 10-17% by weight amine groups.

8. The conjugate according to claim 7 wherein said aminodextran contains 15-17% by weight amine groups.

9. The conjugate according to claim 5 wherein said antibody is an anti-CD3 monoclonal antibody.