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Detection of human papillomavirus by the polymerase chain reaction

  • US 5,527,898 A
  • Filed: 06/07/1995
  • Issued: 06/18/1996
  • Est. Priority Date: 09/09/1988
  • Status: Expired due to Term
First Claim
Patent Images

1. A purified oligonucleotide probe, wherein said probe consists of a nucleotide sequence which hybridizes to a nucleic acid from a human papillomavirus (HPV) type under sequence-specific, stringent hybridization conditions and does not hybridize to a nucleic acid from HPV types 6, 11, 16, 18, and 33 under said conditions, and wherein said probe is chosen from the group consisting ofMYB186 (Seq ID No. 29), MYB187 (Seq ID No. 30), MYB188 (Seq ID No. 31), MYB189 (Seq ID No. 32), MYB202 (Seq ID No. 33), and sequences fully complementary thereto, which hybridize to HPV-26;

  • WD126 (Seq ID No.

         170), WD127 (Seq ID No.

         171), WD128 (Seq ID No.

         172), MY109 (Seq ID No.

         173), MY110 (Seq ID No.

         174)), WD165 (Seq ID No.

         79), WD166 (Seq ID No.

         80) and sequences fully complementary thereto, which hybridize to HPV-31;

    MY92 (Seq ID No.

         175), MY127 (Seq ID No.

         176), MY128 (Seq ID No.

         177), and sequences fully complementary thereto, which hybridize to HPV-31 and HPV-31 B;

    MY115 (Seq ID No.

         181), MY116 (Seq ID No.

         182), MY117 (Seq ID No.

         183), MYB168 (Seq ID No.

         34), MYB169 (Seq ID No.

         35), and sequences fully complementary thereto, which hybridize to HPV-35;

    MY89 (Seq ID No.

         184), MY90 (Seq ID No.

         185), MY91 (Seq ID No.

         186), WD167 (Seq ID No.

         81), WD168 (Seq ID No.

         82), and sequences fully complementary thereto, which hybridize to HPV-39;

    MY118 (Seq ID No.

         187), MY119 (Seq ID No.

         188), MY120 (Seq ID No.

         189), MYB174 (Seq ID No.

         36), MYB175 (Seq ID No.

         37), MYB176 (Seq ID No.

         52), and sequences fully complementary thereto, which hybridize to HPV-40;

    MY33 (Seq ID No.

         192), MY34 (Seq ID No.

         193), MY121 (Seq ID No.

         190), MY122 (Seq ID No.

         122), WD169 (Seq ID No.

         83), and sequences fully, complementary thereto, which hybridize to HPV-42;

    MY68 (Seq ID No.

         194), MY69 (Seq ID No.

         195), MY70 (Seq ID No.

         196), MY98 (Seq ID No.

         197), MY99 (Seq ID No.

         198), MY100 (Seq ID No.

         199), MY108 (Seq ID No.

         200), MY129 (Seq ID No.

         201), WD171 (Seq ID No.

         91), and sequences fully complementary hereto, which hybridize to HPV-45;

    MY87 (Seq ID No.

         202), MY88 (Seq ID No.

         203), and sequences fully complementary thereto, which hybridize HPV-51;

    MY80 (Seq ID No.

         204), MY81 (Seq ID No.

         205), MY82 (Seq ID No.

         206), and sequences fully complementary thereto, which hybridize to HPV-52;

    MY101 (Seq ID No.

         207), MY102 (Seq ID No.

         208), MY103 (Seq ID No.

         209), MYB182 (Seq ID No.

         38), MYB159 (Seq ID No.

         39), and sequences fully complementary thereto, which hybridize to HPV-53;

    MY111 (Seq ID No.

         210), MY112 (Seq ID No.

         211), MY113 (Seq ID No.

         212), MY114 (Seq ID No.

         213), MYB160 (Seq ID No.

         40), MYB161 (Seq ID No.

         41), and sequences fully complementary thereto, which hybridize to HPV-54;

    MYB170 (Seq ID No.

         42), MYB151 (Seq ID No.

         43), MYB171 (Seq ID No.

         44), MY151 (Seq ID No.

         43), MY152 (Seq ID No.

         215), MY153 (Seq ID No.

         216), and sequences fully complementary thereto, which hybridize to HPV-55;

    MYB196 (Seq ID No.

         45), MYB197 (Seq ID No.

         46), MYB198 (Seq ID No.

         47), MYB199 (Seq ID No.

         48), and sequences fully complementary thereto, which hybridize to HPV-56;

    MY154 (Seq ID No.

         217), MY155 (Seq ID No.

         218), MY156 (Seq ID No.

         219), and sequences fully complementary thereto, which hybridize to HPV-57;

    MY93 (Seq ID No.

         220), MY94 (Seq ID No.

         221), MY179 (Seq ID No.

         59), and sequences fully complementary thereto, which hybridize HPV-58;

    MY123 (Seq ID No.

         222), MY124 (Seq ID No.

         223), MYB172 (Seq ID No.

         49), MYB173 (Seq ID No.

         50), MYB162 (Seq ID No.

         51), and sequences fully complementary thereto, which hybridize to HPV-59; and

    MYB191 (Seq ID No.

         66), MYB192 (Seq ID No.

         53), MYB193 (Seq ID No.

         54), MYB194 (Seq ID No.

         67), MYB195 (Seq ID No.

         55), and sequences fully complementary thereto, which hybridize to HPV-68.

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