Potentiometric ion determinations using enhanced selectivity asymmetric ion-selective membranes
First Claim
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1. A process for performing potentiometric ion determinations on biological samples using an ion selective asymmetric membrane electrode and a reference electrode electrolytically connected to the ion-selective asymmetric membrane electrode which comprises:
- (a) contacting a biological sample suspected of containing an ion of interest with the ion-selective asymmetric membrane electrode, the ion-selective asymmetric membrane electrode having an asymmetric membrane with an ion selective layer and a hydrophilic layer fused to the ion-selective layer, the ion-selective layer containing an ion-selective agent and a plasticizing agent, the hydrophilic layer being in contact with the sample,(b) measuring the potential difference between the ion-selective asymmetric membrane electrode and the reference electrode; and
(c) relating the potential difference to the concentration of the ion of interest in the sample, thereby enhancing potentiometric ion selectivity.
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Abstract
This invention relates to a process for performing enhanced selectivity potentiometric ion determinations using an asymmetric ion membrane electrode having an asymmetric membrane with a hydrophilic layer fused to an ion-selective layer.
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19 Claims
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1. A process for performing potentiometric ion determinations on biological samples using an ion selective asymmetric membrane electrode and a reference electrode electrolytically connected to the ion-selective asymmetric membrane electrode which comprises:
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(a) contacting a biological sample suspected of containing an ion of interest with the ion-selective asymmetric membrane electrode, the ion-selective asymmetric membrane electrode having an asymmetric membrane with an ion selective layer and a hydrophilic layer fused to the ion-selective layer, the ion-selective layer containing an ion-selective agent and a plasticizing agent, the hydrophilic layer being in contact with the sample, (b) measuring the potential difference between the ion-selective asymmetric membrane electrode and the reference electrode; and (c) relating the potential difference to the concentration of the ion of interest in the sample, thereby enhancing potentiometric ion selectivity.
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2. A method of using a potentiometric, ion-selective, asymmetric membrane electrode to enhance potentiometric ion selectivity for an ion of interest selected from the group consisting of Cl-, CO32-, and Li+ and to decrease interferences from an interfering ion during the assay of a sample, the method comprising:
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(a) contacting a biological sample with the asymmetric, ion-selective membrane which is in contact within the electrode with a conductor, the asymmetric membrane comprising
1) an ion-selective layer comprising a polymer, an ion-selective agent and a plasticizing agent and
2) a hydrophilic layer fused to the ion-selective layer wherein the hydrophilic layer is in direct contact with the diluted sample;(b) measuring the potential difference between the potentiometric, ion-selective, asymmetric membrane electrode and a reference electrode electrolytically connected to the ion-selective asymmetric membrane electrode in a non-equilibrium or an equilibrium mode; and (c) relating the potential difference to the concentration of the ion chosen in the sample, thereby enhancing potentiometric ion selectivity. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification