Spontaneous cycle fertilization method
First Claim
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1. In an in vitro fertilization method, for human beings, in which one or more oocytes are retrieved, and the one or more oocytes and spermatozoa are introduced into culture medium in a container;
- the container is closed substantially without the introduction of CO2 enriched air and is placed in the vaginal cavity or the uterus for fertilization and culture or culture of the one or more oocytes;
estradiol (E2) and the LH measured by means of blood tests;
the improvement comprising effecting oocyte retrieval without cycle stimulation or injecting chorionic gonadotropin by monitoring E2 levels after the measured LH level is double the baseline LH value, and in response to (i) a first measured LH level which is double the baseline LH value and (ii) a subsequent drop of 30% of the E2 level or relative stability of the E2 level, defined as a change less than about 10% of the previously measured E2 level, performing oocyte retrieval 34 to 36 hours after said first measured LH level.
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Abstract
A fertilization method wherein an oocyte puncture is performed and one or more oocytes are inserted into a container containing a culture medium having no added air or CO2 and intended for the fertilization and culture of the oocyte(s), as well as spermatozoa, whereafter the container is sealed and inserted into the vagina or uterus to allow fertilization of the oocyte(s) and culture of the embryo(s). The method is characterized in that it is performed without stimulating the cycle, with LH supervision by means of blood assays, and by programming the oocyte puncture according to the LH value. The container may be kept at a temperature of approximately 37° C. for 2 to 6 hours for maturation before being inserted into the vagina.
45 Citations
20 Claims
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1. In an in vitro fertilization method, for human beings, in which one or more oocytes are retrieved, and the one or more oocytes and spermatozoa are introduced into culture medium in a container;
- the container is closed substantially without the introduction of CO2 enriched air and is placed in the vaginal cavity or the uterus for fertilization and culture or culture of the one or more oocytes;
estradiol (E2) and the LH measured by means of blood tests;
the improvement comprising effecting oocyte retrieval without cycle stimulation or injecting chorionic gonadotropin by monitoring E2 levels after the measured LH level is double the baseline LH value, and in response to (i) a first measured LH level which is double the baseline LH value and (ii) a subsequent drop of 30% of the E2 level or relative stability of the E2 level, defined as a change less than about 10% of the previously measured E2 level, performing oocyte retrieval 34 to 36 hours after said first measured LH level. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
- the container is closed substantially without the introduction of CO2 enriched air and is placed in the vaginal cavity or the uterus for fertilization and culture or culture of the one or more oocytes;
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2. In an in vitro fertilization method, for human beings, in which one or more oocytes are retrieved, and the one or more oocytes and spermatozoa are introduced into culture medium in a container;
- the container is closed substantially without the introduction of CO2 enriched air and is placed in the vaginal cavity or the uterus for fertilization and culture or culture of the one or more oocytes;
estradiol (E2) and the LH measured by means of blood tests;
the improvement comprising effecting oocyte retrieval without cycle stimulation or injecting chorionic gonadotropin by monitoring E2 levels once the measured LH level is double the baseline LH value, and in response to (i) a first measured LH level double the LH baseline value and (ii) a subsequent 20% increase of the E2 level, performing oocyte retrieval 30 to 34 hours after the measured E2 level with a subsequent 20% increase. - View Dependent Claims (19)
- the container is closed substantially without the introduction of CO2 enriched air and is placed in the vaginal cavity or the uterus for fertilization and culture or culture of the one or more oocytes;
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3. In an in vitro fertilization method, for human beings, in which one or more oocytes are retrieved, and the one or more oocytes and spermatozoa are introduced into culture medium in a container;
- the container is closed substantially without the introduction of CO2 enriched air and is placed in the vaginal cavity or the uterus for fertilization and culture or culture of the one or more oocytes;
estradiol (E2) and the LH measured by means of blood tests;
the improvement comprising effecting oocyte retrieval without cycle stimulation or injecting chorionic gonadotropin by monitoring E2 levels after the measured LH level is double the baseline LH value, and in response to (i) a first measured LH level double the LH baseline value and (ii) subsequent successive measured E2 levels with relative stability, defined as a change less than about 10% of the previous level, performing oocyte retrieval. - View Dependent Claims (20)
- the container is closed substantially without the introduction of CO2 enriched air and is placed in the vaginal cavity or the uterus for fertilization and culture or culture of the one or more oocytes;
Specification
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Current AssigneeMedelle Corporation
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Original AssigneeClaude Ranoux
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InventorsRanoux, Claude
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Primary Examiner(s)Stone, Jacqueline M.
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Assistant Examiner(s)Campell, Bruce R.
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Application NumberUS07/977,409Time in Patent Office1,159 DaysField of Search600/33, 600/34, 600/35, 435/240.2, 435/240.26, 435/975, 800/DIG. 6, 935/99, 935/107, 436/806, 436/906, 436/65US Class Current435/325CPC Class CodesA61B 17/435 for embryo or ova transplan...Y10S 436/906 Fertility tests
