Biotin containing heterobifunctional cleavable compounds

• US 5,532,379 A
• Filed: 05/05/1995
• Issued: 07/02/1996
• Est. Priority Date: 05/05/1995
• Status: Expired due to Term

• Current Assignee: Pierce Biotechnology Incorporated (Perspecta, Inc.)
• Original Assignee: Pierce Biotechnology, Inc. (Perspecta, Inc.)
• Inventors: Fujimoto, Edward K.
• Primary Examiner(s): Richter, Johann
• Assistant Examiner(s): STOCKTON, LAURA LYNNE
• Application Number:
  US08/435,206
• Time in Patent Office: 424 Days
• Field of Search: 548/304.1
• US Class Current: 548/304.1
• CPC Class Codes: C07D 495/04 Ortho-condensed systems

Abstract

Trifunctional cross-linking compounds are disclosed. The compounds contain the biotin moiety, the NHS active ester, and a photoactivatable aryl azide. The presence of a disulfide bond in association with either the ester or azide permits the compound to be cleaved.

Claims

1. A compound having the structure ##STR2## wherein a and b are zero or one, but are different, x is H or SO₃ Na, n is an integer of 1 to 10, each n being the same or different and y is an integer of 1 to 10.

2. A compound of claim 1 wherein a is one and b is zero.

3. A compound of claim 2 wherein x is SO₃ Na.

4. A compound of claim 1 wherein a is zero and b is one.

5. A compound of claim 4 wherein x is SO₃ Na.

6. A compound of claim 1 wherein n is 2.

7. A compound of claim 1 wherein y is 4.

8. A compound of claim 7 wherein n is 2.

9. A compound of claim 8 wherein a is one and b is zero.

10. A compound of claim 9 wherein x is SO₃ Na.

11. A compound of claim 8 wherein a is zero and b is one.

12. A compound of claim 11 wherein x is SO₃ Na.