Sample collection

• US 5,533,516 A
• Filed: 02/22/1995
• Issued: 07/09/1996
• Est. Priority Date: 12/30/1993
• Status: Expired due to Fees

• Current Assignee: Boston Scientific Corporation
• Original Assignee: Boston Scientific Corporation
• Inventors: Sahatjian, Ronald A.
• Primary Examiner(s): HINDENBURG, MAX F
• Application Number:
  US08/392,101
• Time in Patent Office: 503 Days
• Field of Search: 128/749, 128/752, 128/756, 128/757, 604/96, 604/101, 604/194
• US Class Current: 600/562
• CPC Class Codes: A61B 10/0275 with sample notch, e.g. on the side of inner stylet
• CPC Class Codes: A61B 10/04 Endoscopic instruments
• CPC Class Codes: A61B 17/320725 with radially expandable cutting or abrading elements A61B17/32075 takes precedence
• CPC Class Codes: A61B 2017/00292 mounted on or guided by flexible, e.g. catheter-like, means
• CPC Class Codes: A61B 2017/22001 Angioplasty, e.g. PCTA
• CPC Class Codes: A61M 2025/1072 having balloons with two or more compartments
• CPC Class Codes: A61M 2025/1086 having a special balloon surface topography, e.g. pores, protuberances, spikes or grooves
• CPC Class Codes: A61M 2025/109 having balloons for removing solid matters, e.g. by grasping or scraping plaque, thrombus or other matters that obstruct the flow
• CPC Class Codes: A61M 25/10 Balloon catheters A61M25/0125 takes precedence; embolectomy A61B17/22032; retractors A61B17/02; inflatable balloons for placing stents or stent-grafts A61F2/958 ; stomach balloons for treatment of obesity A61F5/0003; oesophagal tubes A61J15/00

Abstract

Taking a bodily sample, e.g., a cell sample, from deep within the body of a patient and collecting the sample outside the body to facilitate treatment of the patient. A sampling probe is provided in the form of an elongate catheter having a proximal portion that remains outside the body and a distal portion that can be located within the body. The distal portion includes a membrane with openings that communicate with space that communicates with a source of suction force. The catheter is positioned within the body. The sample is taken by exposing the membrane by placing it in proximity with a desired location so that the bodily sample is received by the membrane. The catheter is removed from the patient, the sample is collected outside the body, treated, disposed onto a device, such as a graft, stent, or catheter, and reintroduced into the body at a desired site.

Claims

1. A kit for treating a patient by collecting and treating a cell sample from a desired location in the patient, comprising:

a sampling probe in the form of an elongate catheter having a proximal portion that remains outside the body and a distal portion that can be located at the desired location, said distal portion including an expandable member that can be selectively expanded to larger diameters and contracted to smaller diameter,

said expandable member including a membrane on at least a part of an outside surface of said expandable member, said membrane including openings that may receive said cell sample and which communicate with a suction device, controllable from proximal portions outside the body, for creating a suction force through said openings to draw said cell sample into said openings,

an apparatus for facilitating collection and treatment of said cell sample outside the body, and

a device for reintroducing said cell sample into the body.

2. A method for taking a cell sample from a desired location along a blood vessel of a patient and collecting said sample outside the body to facilitate treatment of the patient, comprising:

providing a sampling probe in the form of an elongate vascular catheter having a proximal portion that remains outside the body and a distal portion that can be located in the blood vessel during use, said distal portion including an expandable member that can be selectively expanded to larger diameter and contracted to smaller diameter,

said expandable member including a membrane thereon with openings that communicate with a source of suction force controllable from the proximal portion that remains outside the body,

positioning said expandable portion at a desired location within said blood vessel,

taking said sample by exposing said membrane by expanding said expandable portion to place said membrane in proximity with the wall of said blood vessel so that said sample is received by said membrane,

contracting said expandable portion to said smaller diameter,

removing said catheter from said patient,

collecting said sample outside the body,

treating said cell sample,

disposing the resultant treated cell sample onto a device,

delivering said device on which said treated cell sample is disposed to a desired site in said blood vessel to reintroduce said treated cell sample into the body.

3. The method of claim 2, including drawing said cell sample to the surface of said membrane by suction forces.

4. The method of claim 3, comprising

drawing said sample with said suction forces through said membrane of said sampling probe into a storage space.

5. The method of any one of claims 2, 3 or 4 comprising:

removing said sample by flushing force back out through said openings.

6. The method of any one of claims 2, 3 or 4 comprising:

expanding said expandable portion to contact said membrane with said vessel wall.

7. The method of any one of claims 2, 3 or 4 comprising:

moving said catheter axially while said membrane is in contact with said wall of said vessel to brush said membrane along said wall to collect said sample.

8. The method of any one of claims 2, 3 or 4 comprising:

providing a probe in the form of an angioplasty catheter having an expandable portion in the form of an inflatable balloon, said membrane being disposed over said balloon.

9. The method of claim 8 comprising:

performing angioplasty on an occluded region of said blood vessel,

positioning said expandable portion at said region at said region after said angioplasty, and

collecting said sample from said region.

10. A method for taking a cell sample from deep within the body of a patient and collecting said sample outside the body to facilitate treatment of the patient, comprising:

providing a sampling probe in the form of an elongate catheter having a proximal portion that remains outside the body and a distal portion that can be located within the body, said distal portion including an expandable member that can be selectively expanded to larger diameters and contracted to smaller diameter,

said expandable member including a membrane on at least a part of an outside surface of said expandable member, said membrane including openings that communicate with a suction device, controllable from proximal portions outside the body,

positioning said catheter within the body,

taking said cell sample by expanding said expandable member to place said membrane in proximity to a desired location so that said cell sample is received by said membrane,

removing said catheter from said patient,

collecting said sample outside the body,

treating said cell sample,

disposing the resultant treated cell sample onto a device,

delivering said device on which said treated cell sample is disposed to a desired site in said blood vessel to reintroduce said treated cell sample into the body.

11. The method of claim 2 or 10, wherein said device is a graft or stent.

12. The method of claim 11, wherein said treated cell sample is disposed as an autologous coating on said graft or stent.

13. The method of claim 2 or 10, wherein said device is a catheter.

14. The method of claim 13, wherein said catheter comprises an expandable member including a membrane thereon with openings that communicate with a source of suction force.

15. The method of claim 13, wherein said treated cell sample is disposed on said membrane.

16. The method of claim 15, wherein said treated cell sample is released by a flushing force back out through said openings of said membrane.

17. The method of claim 2 or 10, wherein said treating step comprises culturing said cell sample.

18. The method of claim 2 or 10, wherein said treating step comprises genetically altering said sample.