Process for improving the utilization of feedstuffs by ruminants

• US 5,534,271 A
• Filed: 11/16/1994
• Issued: 07/09/1996
• Est. Priority Date: 11/16/1994
• Status: Expired due to Term

• Current Assignee: Nutrition Physiology Company, LLC (CHR Hansen Holding A/S)
• Original Assignee: Nutrition Physiology Corporation
• Inventors: Ware, Douglas R., Garner, Bryan E.
• Primary Examiner(s): Czaja, Donald E.
• Assistant Examiner(s): Koh, Choon P.
• Application Number:
  US08/340,660
• Time in Patent Office: 601 Days
• Field of Search: 426/2, 426/52, 426/54, 426/61
• US Class Current: 426/2
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/744 Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis

Abstract

A process for improving the utilization of feedstuffs by a ruminant, the process comprising the steps of mixing a lactic acid producing bacteria culture and a lactate utilizing bacteria culture, admixing these cultures with a dry formulation or an animal feedlot diet into a composition, and administering this composition orally to ruminants. The process may be used on a continual basis to increase meat or milk production, or used during the transition from a roughage diet to a feedlot diet to prevent or minimize acidosis. The preferred embodiment utilizes Lactobacillus acidophilus as its lactic acid producing bacteria culture and Propionibacterium P-5 as its lactate utilizing bacteria culture. The composition of the process is in a dry powder form for storage at ambient temperatures for long durations.

Claims

1. A process for improving the utilization of feedstuffs by ruminants through adjusting the microbial content of the rumen of the ruminants, the process comprising the steps of:

mixing a lactic acid producing bacteria culture and a lactate utilizing bacteria culture;

admixing said bacteria cultures with an animal feedlot diet selected from the group consisting of corn, dried grain, alfalfa, corn meal, said admixing forming a composition; and

,

administering said composition orally to the ruminants, said lactic acid producing bacteria culture producing lactic acid in the rumen of the ruminant in order to promote the growth of said lactate utilizing bacteria culture in order to prepare the rumen for a feed diet which generates high levels of lactic acid in the rumen, said lactate utilizing bacteria culture consuming lactic acid produced in the rumen thereby decreasing levels of lactic acid in the rumen.

2. The process according to claim 1 wherein said lactic acid producing bacteria culture is selected from the group consisting of Lactobacillus acidophilus, Lactobacillus plantarum, Streptococcuus faecium, Lactobacillus casei, lactobacillus lactis, Lactobacillus enterii, Lactobacillus fermentum, Lactobacillus delbruckii, Lactobacillus helveticus, Lactobacillus curvatus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus cellobiosuus, Streptococcus lactis, Streptococcus thermophilus, Streptococcus cremoris, Streptococcus diacetylactis, Streptococcus intermedius, Bifidobacterium animalis, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium thermephilum, Pediococcus acidilactici, and Pediococcus pentosaceus.

3. The process according to claim 1 wherein said lactic acid utilizing bacteria culture is selected from the group consisting of Propionibacterium, Propionibacterium freudenreichii, Propionibacterium shermanii, Propionibacterium acidi-propionici, Propionibacterium globosum, Propionibacteriuum jensenii, Peptostreptococcus asaccharolyticus, Megasphaera elsdenii and Selenomonas ruminantium.

4. The process according to claim 2 wherein said Lactobacillus acidophilus is the strain designated as Lactobacillus acidophilus ATCC 53545.

5. The process according to claim 3 wherein said Propionibacterium is the strain selected from the group consisting of Propionibacterium P-99 and Propionibacterium P-42.

6. The process according to claim 3 wherein said Propionibacterium is the strain designated as Propionibacterium P-5.

7. The process according to claim 3 wherein said composition is in a dry powder form for storage at ambient temperatures for long durations.

25. The process of claim 1, further comprising:

producing microbial protein as a product of said lactic acid utilizing bacteria'"'"'s consumption of lactic acid.

26. The process of claim 25, further comprising:

increasing the production of microbial protein by increasing the production of lactic acid available for consumption by said lactic acid utilizing bacteria through the provision of said lactic acid producing bacteria.

8. A process for improving the utilization of feedstuffs by ruminants through adjusting the microbial content of the rumen of the ruminants, the process comprising the step of:

mixing a culture of Lactobacillus acidophilus ATCC 53545, a culture of Propionibacterium P-5 and a culture of Propionibacterium P-99;

admixing said cultures with an animal feedlot diet selected from the group consisting of corn, dried grain, alfalfa, corn meal, said aadmixing forming a composition; and

,

administering said composition orally to the ruminats, said culture of Lactobacillus acidophilus ATCC 53545 producing lactic acid in the rumen of the ruminant in order to promote the growth of said culture of Propionibacterium P-5 and said culture of Propionibacterium P-99 in order to prepare the rumen for a feed diet which generates high levels of lactic acid in the rumen, said culture of Propionibacterium P-5 and said culture of Propionibacterium P-99 consuming lactic acid produced in the rumen thereby decreasing levels of lactic acid in the rumen.

9. The process according to claim 8 wherein said composition is in a dry powder form for storage at ambient temperatures for long durations.

10. A process for improving the utilization of feedstuffs by ruminants through adjusting the microbial content of the rumen of the ruminants, the process comprising the steps of:

mixing a lactic acid producing bacteria culture and a lactate utilizing bacteria culture;

admixing said bacteria cultures with a dry formulation selected from the group consisting of growth substrates, enzymes, sugars, carbohydrates, extracts, and growth promoting micro ingredients, sid admixing forming a composition; and

,

administering said composition orally to the ruminants, said lactic acid producing bacteria culture producing lactic acid in the rumen of the ruminant in order to promote the growth of said lactate utilizing bacteria culture in order to prepare the rumen for a feed diet which generates high levels of lactic acid in the rumen, said lactate utilizing bacteria culture consuming lactic acid produced in the rumen thereby decreasing levels of lactic acid in the rumen.

11. The process according to claim 10 wherein said lactic acid producing bacteria culture is selected from the group consisting of Lactobacillus acidophilus, Lactobacillus plantarum, Streptococcuus faecium, Lactobacillus casei, Lactobacillus lactis, Lactobacillus enterii, Lactobacillus fermentum, Lactobacillus delbruckii, Lactobacillus helveticus, Lactobacillus curvatus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus cellobiosuus, Streptococcus lactis, Streptococcus thermophilus, Streptococcus cremoris, Streptococcus diacetylactis, Streptococcus intermedius, Bifidobacterium animalis, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium thermephilum, Pediococcus acidilactici, and Pediococcus pentosaceus.

12. The process according to claim 10 wherein said lactic acid utilizing bacteria culture is selected from the group consisting of Propionibacterium, Propionibacterium freudenreichii, Propionibacterium shermanii, Propionibacterium acidi-propionici, Propionibacterium globosum, Propionibacteriuum jensenii, Peptostreptococcus asaccharolyticus, Megasphaera elsdenii and Selenomonas ruminantium.

13. The process according to claim 11 wherein said Lactobacillus acidophilus is the strain designated as Lactobacillus acidophilus ATCC 53545.

14. The process according to claim 12 wherein said Propionibacterium is the strain selected from the group consisting of Propionibacterium P-99 and Propionibacterium P-42.

15. The process according to claim 12 wherein said Propionibacterium the strain designated as Propionibacterium P-5.

16. A process for improving the production of meat and milk from ruminants throughout a predetermined feeding period, through adjusting the microbial content of the rumen of the ruminants, the process comprising:

mixing a lactic acid producing bacteria culture and a lactate utilizing bacteria culture;

admixing said bacteria cultures with an animal feedlot diet selected from the group consisting of corn, dried grain, alfalfa, corn meal, said admixing forming a composition; and

,

administering said composition orally to the ruminants on a daily basis during the predetermined feeding period, said lactic acid producing bacteria culture producing lactic acid in the rumen of the ruminant in order to promote the growth of said lactate utilizing bacteria culture in order to prepare the rumen for a feed diet which generates high levels of lactic acid in the rumen, said lactate utilizing bacteria culture consuming lactic acid produced in the rumen thereby decreasing levels of lactic acid in the rumen.

17. The process according to claim 16 wherein said lactic acid producing bacteria culture is selected from the group consisting of Lactobacillus acidophilus, Lactobacillus plantarum, Streptococcuus faecium, Lactobacillus casei, Lactobacillus lactis, Lactobacillus enterii, Lactobacillus fermentum, Lactobacillus delbruckii, Lactobacillus helveticus, Lactobacillus curvatus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus cellobiosuus, Streptococcus lactis, Streptococcus thermophilus, Streptococcus cremoris, Streptococcus diacetylactis, Streptococcus intermedius, Bifidobacterium animalis, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium thermephilum, Pediococcus acidilactici, and Pediococcus pentosaceus.

18. The process according to claim 16 wherein said lactic acid utilizing bacteria culture is selected from the group consisting of Propionibacterium, Propionibacterium freudenreichii, Propionibacterium shermanii, Propionibacterium acidi-propionici, Propionibacterium globosum, Propionibacteriuum jensenii, Peptostreptococcus asaccharolyticus, Megasphaera elsdenii and Selenomonas ruminantium.

19. The process according to claim 17 wherein said Lactobacillus acidophilus is the strain designated as Lactobacillus acidophilus ATCC 53545.

20. The process according to claim 18 wherein said Propionibacterium is the strain is selected from the group consisting of Propionibacterium P-99 and Propionibacterium P-42.

21. The process according to claim 18 wherein said Propionibacterium is the strain designated as Propionibacterium P-5.

22. The process according to claim 16 wherein said composition is in a dry powder form for storage at ambient temperatures for long durations.

23. A process for improving the transition of bovines from a roughage diet to a feedlot diet through adjusting the microbial content of the rumen of the bovine, the process comprising the steps of:

mixing a culture of Lactobacillus acidophilus ATCC 53545, a culture of Propionibacterium P-5 and a culture of Propionibacterium P-99;

admixing said cultures with a dry formulation selected from the group consisting of growth substrates, enzymes, sugars, carbohydrates, extracts, and growth promoting micro ingredients, said admixing forming a composition; and

,

administering said composition orally to the bovines during the transition period, said culture of Lactobacillus acidophilus ATCC 53545 producing lactic acid in the rumen of the ruminant in order to promote the growth of said culture of Propionibacterium P-5 and said culture of Propionibacterium P-99 in order to prepare the rumen for a feed diet which generates high levels of lactic acid in the rumen, said culture of Propionibacterium P-5 and said culture of Propionibacterium P-99 consuming lactic acid produced in the rumen thereby decreasing levels of lactic acid in the rumen.

24. The process according to claim 23 wherein said composition is in a dry powder form for storage at ambient temperatures for long durations.

27. A process for making a feed additive that improves the utilization of feedstuffs that generate high levels of lactic acid by a ruminant, said process comprising the steps of:

providing lactic acid producing bacteria as a ruminant feed additive for increasing lactic acid levels in the rumen of an ingesting ruminant;

providing lactic acid utilizing bacteria as a ruminant feed additive for decreasing lactic acid levels in the rumen of an ingesting ruminant; and

promoting an increase in said lactic acid utilizing bacteria by the provision of said lactic acid producing bacteria.

28. The process of claim 27, further comprising:

producing microbial protein as a product of said lactic acid utilizing bacteria'"'"'s consumption of lactic acid.

29. The process of claim 28, further comprising:

increasing the production of microbial protein by increasing the production of lactic acid available for consumption by said lactic acid utilizing bacteria through the provision of said lactic acid producing bacteria.