Microorganism growth apparatus

• US 5,534,417 A
• Filed: 11/14/1994
• Issued: 07/09/1996
• Est. Priority Date: 06/12/1992
• Status: Expired due to Term

• Current Assignee: Ben Gurion University of The Negev
• Original Assignee: Ben Gurion University of The Negev
• Inventors: Arad, Shoshana (Malis), Cohen, Ephraim
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US08/337,737
• Time in Patent Office: 603 Days
• Field of Search: 435/42, 435/257.1, 435/257.2, 435/67, 435/257.3, 435/257.4, 435/946, 435/284, 435/286, 435/296, 435/311, 435/313-316, 435/813, 435/819, 435/292.1 , 435/296.1, 435/295.2, 47/1.4, 47/1.401, 47/1.402, 47/1.406, 47/1.409
• US Class Current: 435/67
• CPC Class Codes: A01G 33/00 Cultivation of seaweed or algae
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 23/14 Bags
• CPC Class Codes: C12M 23/48 Holding appliances; Racks; Supports holding devices for laboratory apparatus B01L9/00
• CPC Class Codes: C12M 23/58 Reaction vessels connected in series or in parallel combinations of bioreactors with other apparatus, C12M43/00

Abstract

A method of growing microalgae is described, which uses the outdoor sunlight as a source of energy. Growth is confined to an assembly of vertical, transparent tubes through which nutrient and air is carried with carbon dioxide. The microalgae is periodically harvested from the tubes.

Claims

1. A method of growing microalgae outdoors, comprising the steps of:

A) providing a growth apparatus located outdoors,

comprising in combination;

a) a plurality of vertical elongated transparent flexible cells, capable of containing a biomass, the plurality of cells being exposed to daylight;

b) vertical support means to support the plurality of cells in close juxtaposition to one another, and in vertical position;

c) at least one gas inlet and one gas outlet, to permit the bubbling of gas through the biomass in each cell and the ventilation of each cell;

d) at least one fluid inlet and/or fluid outlet to permit feeding of nutrient solution to the biomass and harvesting of the biomass from each cell; and

e) common conveying means connected to each said at least one fluid inlet and/or fluid outlet for harvesting the biomass, to receive and convey biomass harvested from the plurality of cells, all or part of them, away from the growth apparatus;

B) filling the cells of the provided growth apparatus through said at least one fluid inlet and/or fluid outlet with a nutrient solution containing microalgae to be grown;

C) bubbling through said at least one gas inlet and the growth apparatus cells filled with nutrient solution, air in an amount sufficient to obtain efficient mixing of the biomass and removal of oxygen therefrom through said at least one gas outlet;

D) providing to the growth apparatus cell contained biomass, CO₂ in an amount sufficient to promote biomass growth;

E) periodically harvesting from the cells of the growth apparatus the biomass grown by removing a fraction of the volume contained in each cell through said common conveying means, causing solids contained therein to precipitate and recycling the solution from which the solids have precipitated to the cells through said at least one fluid inlet and/or fluid outlet; and

F) making-up the fraction removed from the growth apparatus cells with nutrient solution and/or materials as required.

2. A method according to claim 1, wherein the vertical support means of the apparatus comprise a vertical frame, having at least two vertical elements and one upper horizontal element, the said upper horizontal element being adapted to support the weight of the said plurality of cells.

3. A method according to claim 2, wherein the plurality of cells are provided with connecting means, connectable to the horizontal element of the vertical frame, whereby the said plurality of cells, when connected to said horizontal element, hang therefrom.

4. A method according to claim 2, wherein the perimeter of the horizontal cross-section of each cell is comprised between 5 cm and 100 cm.

5. A method according to claim 2, wherein the height of each cell is comprised between 100 cm and 250 cm.

6. A method according to claim 1, wherein the distance between two adjacent cells is 100 mm or less.

7. A method according to claim 6, wherein each cell is separately connected to the vertical support means.

8. A method according to claim 6, wherein all cells are connected to form a single body.

9. A method according to claim 8, wherein the plurality of cells is created in two layers of weldable material by welding the two layers vertically at intervals.

10. A method according to claim 1, wherein each two adjacent cells are connected by at least one point.

11. A method according to claim 1, wherein said at least one gas inlet comprises a dip pipe for air, the diameter of the said dip pipe being sufficient to permit the flow of an amount of air which efficiently mixes the biomass within each cell.

12. A method according to claim 1, further comprising cooling means to cool the biomass contained within each cell, when a predetermined temperature is exceeded.

13. A method according to claim 12, wherein the cooling means comprise water sprinkling means for sprinkling water along each cell.

14. A method according to claim 1, wherein the cells are made of weldable material.

15. A method according to claim 14, wherein the weldable material is polyethylene.

16. A method according to claim 1, further comprising passages which interconnect between adjacent cells.

17. A method according to claim 1, wherein the bottom portion of each cell is conical in shape, thereby promoting fluidization and mixing and preventing sedimentation of the biomass.

18. A method of producing β

-carotene from β

-carotene-producing microalgae, comprising the steps of;

A) providing a growth apparatus, comprising in combination;

a) a plurality of vertical elongated transparent flexible cells, capable of containing a microalgae biomass;

b) vertical support means to support the plurality of cells in close juxtaposition to one another, and in vertical position;

c) at least one gas inlet and one gas outlet, to permit the bubbling of gas through the biomass in each cell and the ventilation of each cell;

d) at least one cell fluid inlet and/or fluid outlet, to permit feeding of nutrient solution to the biomass and harvesting of the biomass from each cell; and

e) central conveying means connected to each said at least one cell fluid inlet and/or fluid outlet to receive and convey biomass harvested from the plurality of cells, all or part of them, away from the growth apparatus;

B) filling the cells growth apparatus through said at least one cell fluid inlet and/or fluid outlet with a nutrient solution containing biomass of carotene producing microalgae to be grown;

C) bubbling through said at least one gas inlet of the cell filled with nutrient solution, air in an amount sufficient to obtain efficient mixing of the biomass and removal of oxygen therefrom through said at least one gas outlet;

D) providing to the growth apparatus cell contained biomass, CO₂ in an amount sufficient to promote biomass growth;

E) periodically harvesting from the cell of the growth apparatus, the biomass grown by removing a fraction of the volume contained in each cell through said common conveying means, causing solids contained therein to precipitate and recycling the solution from which the solids have precipitated to the cells through said at least one fluid inlet and/or fluid outlet;

F) making-up the fraction removed from the cells with nutrient solution and/or materials as required; and

G) recovering β

-carotene from the harvested biomass.

19. A method according to claim 18, wherein the make-up nutrient solution of step F consists at least partially of recycled nutrient solution from the biomass separation step.

20. A method according to claim 18, wherein CO₂ is provided together with the air.

21. A method according to claim 20, wherein the content of CO₂ in the air is about 2-3%.

22. A method according to claim 18, wherein the microalgae is Dunaliella sp.