Basal nutrient medium for in vitro cultures of loblolly pines
First Claim
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1. An aqueous basal nutrient medium for loblolly pine tissue cultures which comprises:
- 15-30 mM of NO3, 6-15 mM of NH4, 10-25 mM of K, 1-2.3 mM of H2 PO4, 2-7 mM of Ca, 1-9 mM of Mg, 1-10 mM of SO4, 0.0001-25 mM of Cl, 0.1-0.3 mM of Na, 0.05-0.6 mM of H2 BO4, 0.45-0.124 mM of Mn, 0.05-0.2 mM of Fe, 0.015-0.2 mM of Zn, 0.0005-0.003 mM of Cu, 0.0001-0.001 mM of I, 0.00005-0.006 mM of MoO4, 0.00005-0.006 mM of Co, 0.0002-0.002 mM of Thiamine, and 0.05-0.2 mM of ethylenediaminetetraacetic acid.
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Abstract
A basal nutrient culture medium for the in vitro culture of Pinus taeda, loblolly pine, is disclosed. The medium contains nitrate, ammonium, potassium, phosphorate, calcium, magnesium, sulfate, chlorine, sodium, borate, manganese, iron, zinc, copper, iodine, molybdenum oxide, cobalt, thiamine and EDTA.
38 Citations
20 Claims
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1. An aqueous basal nutrient medium for loblolly pine tissue cultures which comprises:
- 15-30 mM of NO3, 6-15 mM of NH4, 10-25 mM of K, 1-2.3 mM of H2 PO4, 2-7 mM of Ca, 1-9 mM of Mg, 1-10 mM of SO4, 0.0001-25 mM of Cl, 0.1-0.3 mM of Na, 0.05-0.6 mM of H2 BO4, 0.45-0.124 mM of Mn, 0.05-0.2 mM of Fe, 0.015-0.2 mM of Zn, 0.0005-0.003 mM of Cu, 0.0001-0.001 mM of I, 0.00005-0.006 mM of MoO4, 0.00005-0.006 mM of Co, 0.0002-0.002 mM of Thiamine, and 0.05-0.2 mM of ethylenediaminetetraacetic acid.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Current AssigneeMeadwestvaco (WestRock Company)
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Original AssigneeWestvaco Corporation (Meadwestvaco Corporation)
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InventorsCoke, Jay E.
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Primary Examiner(s)Naff, David M.
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Assistant Examiner(s)LANKFORD JR, LEON B
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Application NumberUS08/457,876Time in Patent Office403 DaysField of Search435/240.4, 435/240.54, 435/240.45US Class Current435/431CPC Class CodesC12N 5/0025 Culture media for plant cel...
