Isolated DNA of human papillomavirus type 55 (HPV55)

• US 5,534,439 A
• Filed: 05/10/1994
• Issued: 07/09/1996
• Est. Priority Date: 05/13/1988
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Institut Pasteur
• Inventors: Kremsdorf, Dina, Orth, Gerard, Pehau-Arnaudet, Gerard, Favre, Michel
• Primary Examiner(s): Nucker, Christine M.
• Assistant Examiner(s): SCHEINER, LAURIE A
• Application Number:
  US08/240,497
• Time in Patent Office: 791 Days
• Field of Search: 435/5, 435/172.3, 435/235.1, 435/252.3, 435/320.1, 536/23.1, 536/23.74, 436/64
• US Class Current: 435/320.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/084 Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
• CPC Class Codes: C12N 2710/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/708 for papilloma

Abstract

The invention relates to papillomavirus probes derived from new DNA-HPVs deposited with the C.N.C.M. on May 6, 1988, under the following filing numbers:

______________________________________                                    

PGEM 4 HPV49: I-754 PSP 65 HPV50: I-755 PSP 64 HPV54: I-756 PGEM 4 HPV55A: I-757 PGEM 4 HPV55B: I-758 ______________________________________

These probes can be used for in vitro detection of:

in the case of HPV49: warts of the skin (in particular, common and plantar warts) and the differential diagnosis of epidermodysplasia verruciformis,

in the case of HPV50: epidermodysplasia verruciformis, intraepithelial neoplasias and cutaneous cancer,

in the case of HPV55_-- : genital neoplasias and cancers of the uterine cervix,

in the case of HPV55_-- : genital neoplasias and cancers of the uterine cervix, condylomas and papillomas.

Claims

1. Isolated and essentially pure DNA having the nucleotide residue sequence of HPV 55 having Accession Numbers C.N.C.M. I-757 and I-758.

2. The isolated and essentially pure HPV 55 DNA of claim 1, wherein said DNA is labelled with a label selected from the group consisting of a radioactive marker and a non-radioactive marker.

3. A recombinant DNA comprising a cloning vector and HPV 55 DNA of claim 1.