Emulsions as contrast agents and method of use

• US 5,536,489 A
• Filed: 05/12/1995
• Issued: 07/16/1996
• Est. Priority Date: 06/04/1993
• Status: Expired due to Fees

• Current Assignee: Molecular Biosystems, Inc (Alliance Pharmaceutical Corporation)
• Original Assignee: Molecular Biosystems, Inc (Alliance Pharmaceutical Corporation)
• Inventors: Krishnan, Ashwin M., Lohrmann, Rolf, Widder, Kenneth J., Meng, Jialun, Hong, Dung K.
• Primary Examiner(s): Hollinden, Gary E.
• Application Number:
  US08/439,693
• Time in Patent Office: 431 Days
• Field of Search: 424/9.52, 252/312, 128/662.02
• US Class Current: 424/9.52
• CPC Class Codes: A61K 49/223 Microbubbles, hollow microspheres, free gas bubbles, gas microspheres
• CPC Class Codes: A61K 49/226 Solutes, emulsions, suspensions, dispersions, semi-solid forms, e.g. hydrogels
• CPC Class Codes: Y10S 516/929 Specified combination of agitation steps, e.g. mixing to make subcombination composition followed by homogenization

Abstract

This invention relates to an oil-in-water emulsion that is of a water-insoluble gas-forming chemical and a stabilizer. The emulsion being capable of forming microbubbles of gas upon application of ultrasonic energy. This composition allows for site specific imaging as the image enhancing microbubbles can be released upon the application of ultrasonic energy at the specific location where the image is desired.

Claims

1. A method to enhance the contrast of tissues and organs in an ultrasonic image comprising:

(a) injecting an emulsion comprising at least one water-insoluble gas-forming chemical and at least one stabilizer into a patient, said emulsion being capable of forming microbubbles of gas upon application of ultrasonic energy (b) applying a sufficient amount of ultrasonic energy to volatilize said chemicals to release microbubbles; and

(c) detecting an ultrasonic image.

2. The method of claim 1 wherein said stabilizer is a hydrophobic compound.

3. The method of claim 2 wherein said emulsion includes a surface active agent.

4. The method of claim 1 wherein said stabilizer is amphipathic.

5. The method of claim 4 wherein said amphipathic compound is:

##STR3## wherein the average value of n=75 and the average value of b=30 such that the average molecular weight of said compound is 8350.

6. The method of claim 1 wherein said emulsion includes a viscosifier.

7. The method of claim 1 wherein said emulsion includes antioxidants.

8. The method of claim 1 wherein said emulsion includes a component that imparts tissue specificity to said emulsion.

9. The method of claim 1 wherein said emulsion includes a component that imparts organ specificity to said emulsion.

10. The method of claim 1 wherein said water-insoluble gas-forming chemical is nonafluoro-t-butylmethane.

11. The method of claim 1 wherein said water-insoluble gas forming chemical is perfluoropentane and said stabilizer is lecithin.