Aminoalkylphosphorothioamidate oligonucleotide deratives

• US 5,536,821 A
• Filed: 12/16/1994
• Issued: 07/16/1996
• Est. Priority Date: 03/08/1990
• Status: Expired due to Term

• Current Assignee: Worcester Foundation For Biomedical Research
• Original Assignee: Worcester Foundation For Biomedical Research
• Inventors: Agrawal, Sudhir, Tang, Jin-Yan
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): TRAN, PAUL
• Application Number:
  US08/357,399
• Time in Patent Office: 578 Days
• Field of Search: 536/24.3, 536/25.33, 536/26.5, 536/25.6, 536/26.6, 536/22.1, 536/23.1, 435/6
• US Class Current: 536/22.1
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Disclosed are methods of producing a synthetic oligonucleotide of selected nucleotide sequence which is internally functionalized at at least one selected location with an aminoalkylphosphorothioamidate and labelled with a nonradioactive material. Also disclosed are oligonucleotides produced by this method.

Claims

1. A compound consisting of a range of five to fifty covalently linked nucleosides wherein at least one linkage between the nucleosides is through an aminoalkylphosphorothioamidate residue having the structure:

##STR7## wherein n is 2 to 6, and Nu₁ and Nu₂ are those nucleosides adjacent the residue.

2. The compound according to claim 1, wherein the nucleosides are selected from the group consisting of adenosine, thymidine, cytidine, guanosine, and uridine.