Hepatitis C virus epitopes

• US 5,538,865 A
• Filed: 01/19/1994
• Issued: 07/23/1996
• Est. Priority Date: 04/06/1990
• Status: Expired due to Term

• Current Assignee: GlaxoSmithKline LLC (GlaxoSmithKline PLC)
• Original Assignee: Genelabs Technologies, Inc. (GlaxoSmithKline PLC)
• Inventors: Reyes, Gregory, Kim, Jungsuh P., Moeckli, Randolph
• Primary Examiner(s): Woodward, Michael P.
• Application Number:
  US08/184,236
• Time in Patent Office: 916 Days
• Field of Search: 435/5, 435/69.3, 530/350
• US Class Current: 435/69.3
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2770/24222 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: Y10S 530/826 Viruses

Abstract

Peptide antigens which are immunoreactive with sera from individuals infected with hepatitis C virus (HCV) are disclosed. Several of the antigens are immunologically reactive with antibodies present in individuals identified as having chronic and acute HCV infection. The antigens are useful in diagnostic methods for detecting HCV infection in humans. Also disclosed are corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic peptides.

Claims

1. A purified polypeptide immunoreactive with sera from humans infected with hepatitis C virus (HCV), which:

a) is encoded by an HCV coding sequence; and

b) includes a 504 amino acid polypeptide encoded by said HCV sequence having the sequence presented as SEQ. ID NO;

6.

2. A polypeptide for detecting the presence of Hepatitis C Virus antibodies in a serum sample, comprising

a polypeptide produced by bacterial cells containing lambda vector ATCC No. 40901, where said polypeptide contains the sequence presented as SEQ ID NO:

4.