Nucleic acids encoding a TGF-.beta. type 1 receptor

• US 5,538,892 A
• Filed: 11/04/1993
• Issued: 07/23/1996
• Est. Priority Date: 03/18/1992
• Status: Expired due to Term

• Current Assignee: Duke University, The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: Duke University, The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Gustafson, Michael, Wang, Xiao-Fan, He, Wei-Wu, Donahoe, Patricia K.
• Primary Examiner(s): Walsh, Stephen G.
• Assistant Examiner(s): Fitzgerald, David L.
• Application Number:
  US08/149,105
• Time in Patent Office: 992 Days
• Field of Search: 536/23.5, 536/24.31, 435/240.2, 435/320.1, 435/69.1, 435/252.3, 435/254.11
• US Class Current: 435/354
• CPC Class Codes: A61K 47/6849 the antibody targeting a receptor, a cell surface antigen or a cell surface determinant
• CPC Class Codes: C07K 14/71 for growth factors; for growth regulators
• CPC Class Codes: C07K 14/72 for hormones for neuromediators C07K14/70571
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants

Abstract

Isolated DNAs (e.g., cDNAs or genomic fragments) encoding TGF-β type I receptors, or soluble, ligand-binding fragments thereof; vectors or cells which contain such DNAs; and substantially pure polypeptides encoded by such DNAs, whether produced by expression of the isolated DNAs, by isolation from natural sources, or by chemical synthesis.

Claims

1. Isolated DNA comprising a nucleotide sequence encoding a transforming growth factor-beta (TGF-β

) type I receptor having the amino acid sequence of a receptor endogenously expressed in a mammalian tissue, wherein said nucleotide sequence hybridizes under high stringency conditions with a 50 nucleotide portion of either strand of misr4 (SEQ ID NO;

     4).

2. The isolated DNA of claim 1, wherein said receptor is a human protein.

3. The isolated DNA of claim 1, wherein said receptor is a mouse protein.

4. The isolated DNA of claim 1, wherein said receptor is a rat protein.

5. The isolated DNA of claim 4, wherein said receptor has at least 70% amino acid sequence identity with the amino acid sequence of MISR4 (SEQ ID NO:

17).

6. The isolated DNA of claim 4, wherein said receptor has the amino acid sequence of MISR4 (SEQ ID NO:

17).

7. The isolated DNA of claim 1, wherein said sequence encoding said receptor hybridizes under high stringency conditions with the coding sequence of misr4 (SEQ ID NO:

4).

8. Isolated DNA comprising a nucleotide sequence encoding the receptor encoded by the isolated DNA of claim 1, wherein said receptor is a human TGF-β

type I receptor.

9. Isolated DNA comprising a nucleotide sequence encoding the receptor encoded by the isolated DNA of claim 1, wherein said receptor is a rat TGF-β

type I receptor.

10. Isolated DNA comprising a nucleotide sequence encoding the receptor encoded by the isolated DNA of claim 1, wherein said receptor is a mouse TGF-β

type I receptor.

11. A cell comprising the isolated DNA of claim 1.

12. The cell of claim 8, wherein said cell is capable of expressing said receptor.

13. The cell of claim 11, wherein said cell is a eukaryotic cell.

14. The cell of claim 11, wherein said receptor is a human protein.

15. The isolated DNA of claim 1, wherein said sequence encoding said receptor is under the transcriptional control of a heterologous promoter.

16. A vector comprising the isolated DNA of claim 1.

17. The vector of claim 16, wherein said vector is a viral nucleic acid.

18. The vector of claim 16, wherein said receptor is a human protein.

19. A substantially pure nucleic acid at least 50 nucleotides in length having the nucleotide sequence of a fragment of the isolated DNA of claim 1.

20. The nucleic acid of claim 19, wherein said nucleic acid is incorporated into a vector.

21. The nucleic acid of claim 19, wherein said receptor is a human protein.