Multi-well titerplate for instrumental analysis

• US 5,540,891 A
• Filed: 10/11/1994
• Issued: 07/30/1996
• Est. Priority Date: 10/18/1993
• Status: Expired due to Fees

• Current Assignee: Schweizerische Eidgenossenschaft Vertreten Durch Das Ac-Laboratorium Spiez Der Gruppe Rustung
• Original Assignee: Scheizerische Eidgenossenschaft Vertreten Durch Das Ac-Laboratorium Spiez Der Gruppe Fur Rustungsdienste
• Inventors: Portmann, Rudolf, Hirschi, Andreas, Wellenreiter, Andreas
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Pyon, Harold Y.
• Application Number:
  US08/321,395
• Time in Patent Office: 658 Days
• Field of Search: 422/101, 422/102, 422/99, 220/528, 220/529, 220/533, 220/527, 220/507, 220/611, 220/610, 220/507, 206/443, 206/446, 211/74, 435/301, 356/244, 356/246, 264/138, 264/219, 264/333
• US Class Current: 422/553
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/03 Cuvette constructions

Abstract

A titerplate for instrumental analysis of liquid samples consists of a plurality of wells in which the walls and bottoms are made of two different materials with different physical properties. This allows the interfacial tension at the surface of the liquid to be controlled in such a way that it is essentially planar, allowing more accurate analysis to be performed on the samples. The bottoms of the wells are preferably made of a transparent material, so that the analysis and observation can be done in the transmission mode.

Claims

1. A multi-well titerplate assembly for use in conjunction with instrumental analysis is of liquid samples, comprising a plurality of individual wells each having a side wall and a bottom, said bottoms being arrayed in a common plane and being formed as a series of elevations upon a bottom plate, said side wall being of an apolar thermoplastic material chosen to interact with a liquid sample to be placed within the well whereby the resulting interfacial tension between the sample and the well side wall results in a generally planar sample surface, the material for the well bottom being a transparent thermoplastic material chosen to allow observation and analysis of the sample therethrough, the side wall being mounted with a friction-fit about the periphery of the wall bottom.

2. The assembly of claim 1, wherein the well wall material is chosen from the group consisting of polyolefin or polytetrafluoroethylene and the well bottoms are constructed of a material chosen from the group consisting of polystyrene, polystyrene co-polymer, polymethylmethacrylate, polycarbonate, polyethersulfone, polysulfone, polysulfone, polymethylpentene, styreneacrylnitrile, amorphous polyamid and polymethylentherephtalate.

3. The apparatus according to claim 1, wherein at least one of the wall and bottom of a well comprise a sample-adsorbing surface.

4. The apparatus according to claim 1, wherein at least two of said well walls are mechanically connected to form an individual sub-assembly unit.

5. The apparatus of claim 1 further comprising means associated with each of the wells to identify each well and samples placed therein.

6. The apparatus according to claim 5, wherein said means comprise a common protective cover.

7. The apparatus of claim 1, further comprising at least one alignment shaft extending vertically upward from said bottom plate, wherein said individual sub-assembly element includes at least one spigot hole for mounting upon one of said alignment shafts.

8. The apparatus of claim 7, wherein said sub-assembly unit comprises a web portion joining the well walls, said at least one spigot hole being located in said web, said web being located vertically along said well walls to vertically align said sub-assembly unit with respect to said elevations when an end of said alignment shaft is co-planar with a top surface of said web.

9. The apparatus of claim 8 further comprising a top plate and a peripheral depending lip, said top plate having a series of holes therein each located and dimensioned to accept a top portion of a well wall.

10. The apparatus of claim 9, wherein said top surface of said web is adapted to support said top plate.