Method of control of microorganism growth process

• US 5,541,056 A
• Filed: 07/22/1994
• Issued: 07/30/1996
• Est. Priority Date: 10/10/1989
• Status: Expired due to Term

• Current Assignee: Aquasearch, Inc. (Mera Pharmaceuticals Incorporated)
• Original Assignee: Aquasearch, Inc. (Mera Pharmaceuticals Incorporated)
• Inventors: Redalje, Donald, Niiler, Pearn P., Huntley, Mark E.
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US08/279,740
• Time in Patent Office: 739 Days
• Field of Search: 435/3, 435/41, 435/240.1, 435/240.4, 435/240.46, 435/240.47, 435/257.1, 435/257.3, 435/257.6, 435/284, 435/286, 435/289, 435/316, 435/286.1 , 435/286.5, 435/289.1, 435/292.1, 435/293.1, 435/298.1, 47/1.4
• US Class Current: 435/3
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 21/04 for producing gas, e.g. biogas digesters for manure with production of biogas A01C3/028, biological treatment of water, waste water or sewage C02F3/00, C02F11/02, preparation of natural gas or syngas C10L3/06, C10L3/10
• CPC Class Codes: C12M 29/02 Percolation
• CPC Class Codes: C12M 33/10 by centrifugation centrifuges in general B04B; Cyclones cyclones in general B04C
• CPC Class Codes: C12M 33/22 Settling tanks; Sedimentation by gravity settling tanks per se B01D21/02
• CPC Class Codes: C12M 41/00 Means for regulation, monitoring, measurement or control, e.g. flow regulation controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N
• CPC Class Codes: C12M 41/06 of illumination
• CPC Class Codes: C12M 47/10 Separation or concentration of fermentation products bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02
• CPC Class Codes: C12N 1/12 Unicellular algae; Culture media therefor as new plants A01H13/00

Abstract

A control method for operating aqueous microorganism growth processes is disclosed which can maintain viable growth conditions for many types of microorganisms which have heretofore not been easily reproduced in commercially valuable quantities. The primary control parameters are the degree of turbulence in the aqueous growth medium and the scale of the apparatus relative to the scale of the turbulent eddies in vessels which are partially filled with the aqueous medium directly affect conditions which are required for optimum growth: light exposure, nutrient supply, sedimentation rate, bulk temperature, gas exchange rate and cell integrity. These control elements can be cast in terms of the Reynolds number (N_Re) and controlled dimensions of the apparatus (L_K) in relationship to the scales of turbulent eddies to define the dissipation λ_K as L_K /L. Alternatively, these can be recognized to show that the delivery of nutrients to the microorganism mass is dependent upon the dissipation rate ε in the fluid, which allows a definition of L_k to be quantified. The broadest ranges generally useful in this invention are N_Re =2000 to 10⁶, preferably 4000 to 250,000; apparatus scale relative to eddy scale is such that the level of fluid in the reaction chamber relative to eddy scale is 10-1:1 preferably 10-1.2:1; and turbulent energy dissipation rate ε between 10^-3 and 10 W/kg. The invention is particularly useful in controlling growth processes in which the microorganisms are unicellular algae, phytoplankton, issues of vascular plants, tissues of macroalgae, and the like.

Claims

1. A method for the control of an aqueous microorganism growth process which comprises:

distributing a photosynthetic microbial biomass in an aqueous medium within a reaction chamber, said reaction chamber being transparent to visible light, and said aqueous medium containing a suspension of microbial biomass occupying less than the total volume of said chamber;

maintaining said biomass within said reaction chamber for a period of time during which microorganisms comprising said biomass reproduce and increase in number;

providing nutrients to and removing evolved gases from said biomass during said period of time to support said reproduction;

flowing quantities of said aqueous medium through said reaction chamber throughout said time period;

subjecting said reaction chamber to a source of visible light for at least a portion of said period of time with said visible light passing into said reaction chamber and being used by said microorganisms to enable photosynthesis;

maintaining a turbulent flow regime with turbulent eddies predominantly throughout said aqueous medium within said reaction chamber for said period of time;

maintaining a predetermined range of ratios between scale of said chamber and scale of said eddies for said period of time;

such that in said turbulent flow regime effective reaction conditions are maintained for said reproduction of said microorganisms until said time period has been sufficient to produce a desired increase in number of said microorganisms.

2. A method as in claim 1 wherein said turbulent flow regime is defined by a Reynolds Number value of between 2000 and 10⁶.

3. A method as in claim 2 wherein said turbulent flow regime is defined by a Reynolds Number value of between 4000 and 10⁶.

4. A method as in claim 3 wherein said turbulent flow regime is defined by a Reynolds Number value of between 4000 and 250,000.

5. A method as in claim 4 wherein said turbulent flow regime is defined by a Reynolds Number value of between 5000 and 50,000.

6. A method as in claim 1 wherein said range of ratios is 10-1:

1.

7. A method as in claim 6 wherein said range is 10-1.2:

1.

8. A method as in claim 1 wherein said microbial biomass comprises at least one type of microorganism from the group selected from unicellular phytoplankton, tissues of vascular plants and tissues of macroalgae.

9. A method as in claim 8 wherein said microorganism or tissues of the aforementioned plants is selected from the group consisting of Dinophyceae, Bacillariophyceae, Lyngbya lagerheimii (Cyanophyceae), Spirulina platensis (Cyanophyceae), Haematococcus pluvialis (Chlorophyceae), Chlorella sorokiniensis (Chlorophyceae), and plant cell tissue cultures.

10. A method as in claim 9 wherein said microorganism is selected from the group consisting of Haematococcus pluvialis, Spirulina platensis, Lyngbya lagerheimii, and Chlorella sorokiniensis.

11. A method as in claim 1 wherein said reaction conditions in said reaction chamber comprise light exposure, nutrient supply, sedimentation rate, bulk temperature, gas exchange rate and cell integrity.

12. A method as in claim 1 wherein said turbulent flow regime causes movement of said microorganisms within said biomass such that exposure of individual microorganisms to said visible light is intermittent.

13. A method as in claim 12 wherein said intermittent exposure of visible light to said microorganisms creates a flashing light effect on the photosynthesis produced in said microorganisms.

14. A method as in claim 13 wherein said microorganisms are exposed to said visible light for a cumulative time of approximately ten times longer than the cumulative time of non-exposure.

15. A method as in claim 1 further comprising maintaining a turbulent energy dissipation rate ε

between 10^-3 and 10 W/kg.

16. A method as in claim 1 wherein said aqueous medium containing said microbial mass occupies approximately 30%-90% of the total volume of said chamber.