Production of interferon

• US 5,541,312 A
• Filed: 09/28/1982
• Issued: 07/30/1996
• Est. Priority Date: 11/21/1979
• Status: Expired due to Term

• Current Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Original Assignee: Yeda Research and Development Co. Ltd. (Weizmann Institute of Science)
• Inventors: Revel, Michel, Tiollais, Pierre
• Primary Examiner(s): Martinell, James
• Application Number:
  US06/425,934
• Time in Patent Office: 5,054 Days
• Field of Search: 435/68, 435/70, 435/91, 435/172, 435/253, 435/317, 435/69.51, 435/91.1, 435/172.3, 435/320.1, 435/252.3-252.35, 435/811, 536/27, 536/23.52, 935/13, 935/21, 530/351
• US Class Current: 536/23.52
• CPC Class Codes: C07K 14/5412 IL-6
• CPC Class Codes: C07K 14/565 IFN-beta

Abstract

The present invention .relates to a process to isolate genetic material (DNA) containing the nucleotide sequence coding for interferon in human fibroblastic cells which comprises cultivating cells producing interferon when exposed to an inducer of interferon, exposing same to such inducer, extracting messenger RNA from said induced cells, purifying the interferon messenger RNA, transcribing the messenger RNA into DNA and cloning the DNA in a suitable vector. Preferred cells are human diploid foreskin cells. The invention further relates to a process for engineering a bacterial strain to produce interferon polypeptide which comprises introducing a cloned interferon DNA into a suitable vector-carrier. A preferred vector-carrier is E. coli. The invention also relates to the mRNA of human interferon in highly purified form, to the mRNA of human interferon in β1 highly purified form, to the mRNA of human interferon in ⊕2 highly purified form, to the DNA coding for a polypeptide having interferon activity, insertable in a vector, such as plasmid pBR322, and also to human interferon β1 in highly purified form, and human interferon β2 in highly purified form.

Claims

1. A DNA which consists essentially of a DNA which codes for human fibroblast β

-2 interferon polypeptide.

2. A vector which consists essentially of a vector containing genetic material including a nucleotide sequence translatable to a human fibroblast interferon-beta-2 polypeptide.

3. A heat cell which consists essentially of a cultivatable vector having a DNA insert which codes for a human fibroblast interferon-beta-2 polypeptide.

4. A vector which consists essentially of a vector containing a DNA molecule including a portion of the DNA which codes for interferon-beta-2, which DNA molecule is capable of hybridizing to mRNA encoding human interferon-beta-2 in 50% formamide at 52°

.

5. A host cell which consists essentially of a cultivatable vector in accordance with claim 4.

6. A vector in accordance with claim 4, wherein said portion of the DNA which codes for interferon-beta-2 is that portion which is present in cDNA clone A341, CNCM Depository Accession No. 1-1214.

7. A host cell which consists essentially of a cultivatable vector in accordance with claim 6.