Method for diagnosing myofibrogranuloma (MFG) in walleye

• US 5,543,330 A
• Filed: 09/22/1995
• Issued: 08/06/1996
• Est. Priority Date: 09/22/1995
• Status: Expired due to Fees

• Current Assignee: Center For Innovation & Business Development Foundation
• Original Assignee: Center For Innovation & Business Development Foundation
• Inventors: Shoemaker, Craig A., Holloway, Harry L. Jr.
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/532,091
• Time in Patent Office: 319 Days
• Field of Search: 436/63, 436/79, 436/98, 436/811, 119/215, 119/216, 119/217
• US Class Current: 436/79
• CPC Class Codes: G01N 33/12 Meat; fish
• CPC Class Codes: G01N 33/70 involving creatine or creatinine
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function
• CPC Class Codes: Y10T 436/147777 Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]

Abstract

An improved method for diagnosing myofibrogranuloma (MFG) in walleye without harming the fish is described. Blood samples are drawn from walleye and creatinine and calcium levels are then determined along with the sex and length of the fish. These data are then analyzed and compared to data from healthy walleye using discriminant analysis. The process is effective for identifying approximately 88% of MFG-positive walleye.

Claims

1. A method of detecting myofibrogranuloma (MFG) in walleye, wherein the walleye is greater than or equal to 500 mm total length, comprising:

determining the serum creatinine value in a walleye;

determining the serum calcium value in the walleye; and

performing a discriminant analysis utilizing the serum creatinine value and serum calcium value as quantitative variables.

2. The method of claim 1 wherein the mean serum creatinine level for MFG-positive walleye is 0.34 mg/dl.

3. The method of claim 1 wherein the serum calcium level for MFG-positive male walleye is within the range of 11.49-16.80 mg/100 ml and the serum calcium level for MFG-positive female walleye is within the range of 17.12-25.35 mg/100 ml.

4. The method of claim 3 wherein the mean serum calcium level for MFG-positive male walleye is 13.62 mg/100 ml and the mean serum calcium level for MFG-positive female walleye is 20.20 mg/100 ml.

5. The method of claim 1 wherein the discriminant analysis uses the variables sex, length, creatinine and calcium.

6. The method of claim 1 wherein about 88% of MFG-positive walleye are recognized.

7. An improved method for detecting myofibrogranuloma (MFG) in walleye comprising:

measuring the serum calcium level in a walleye;

measuring the serum creatinine level in the walleye;

evaluating the serum calcium level and serum creatinine level in conjunction with the mean serum calcium and creatinine levels from MFG-positive walleye and healthy walleye using discriminant analysis.

8. The improved method of claim 7 wherein the walleye is greater than or equal to 500 mm in total length.

9. The improved method of claim 7 wherein the mean serum calcium level for MFG-positive male walleye is 13.62 mg/100 ml and the mean serum calcium level for MFG-positive female walleye is 20.20 mg/100 ml.

10. The improved method of claim 7 wherein the mean serum creatinine level for MFG-positive walleye is 0.34 mg/dl.

11. The improved method of claim 7 wherein the mean serum calcium level for healthy male walleye is 11.68 mg/100 ml and the mean serum calcium level for healthy female walleye is 15.36 mg/100 ml.

12. The improved method of claim 7 wherein the mean serum creatinine level for healthy walleye is 0.283 mg/dl.