Liquid column packing materials and method for making the same
First Claim
1. A method for making a packing material for liquid chromatographic or catalytic columns which comprises:
- a) providing a porous protein-adsorptive support,b) providing an aqueous solution into which a protein has been dissolved,c) contacting said porous protein-adsorptive support with said aqueous solution into which a protein has been dissolved for a sufficient period of time to form, and under conditions which form, a saturated coating of protein on the external surfaces of said porous protein-adsorptive support,d) removing excess protein that remains in solution, and,e) crosslinking said saturated coating of protein in situ to form a relatively thin but saturated coating of crosslinked protein on the external surfaces of said porous protein-adsorptive support wherein said relatively thin but saturated coating of crosslinked protein gives a signal obtained by at least one surface selective analytical method for nitrogen which does not exceed 67 percent of that for a bulk amount of the crosslinked protein.
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Abstract
Packing materials for liquid chromatographic or catalytic columns are prepared by contacting a porous protein-adsorptive particulate or membranous support, such as a porous silica particulate support, with an aqueous solution into which a protein has been dissolved to form a saturated coating of protein on the external surfaces of the porous protein-adsorptive support, removing excess protein that remains in solution by washing, and, then crosslinking the protein in the coating. The result is a packing material which resists further adsorption by many different proteins but which continues to provide the adsorptive or catalytic properties of the groups on the internal surfaces of the porous protein-adsorptive support for separations, analysis, or alteration of small molecules. The packing material of the present invention is particularly useful in HPLC or solid phase extraction columns for direct injection drug analysis in plasma, serum, and urine.
17 Citations
13 Claims
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1. A method for making a packing material for liquid chromatographic or catalytic columns which comprises:
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a) providing a porous protein-adsorptive support, b) providing an aqueous solution into which a protein has been dissolved, c) contacting said porous protein-adsorptive support with said aqueous solution into which a protein has been dissolved for a sufficient period of time to form, and under conditions which form, a saturated coating of protein on the external surfaces of said porous protein-adsorptive support, d) removing excess protein that remains in solution, and, e) crosslinking said saturated coating of protein in situ to form a relatively thin but saturated coating of crosslinked protein on the external surfaces of said porous protein-adsorptive support wherein said relatively thin but saturated coating of crosslinked protein gives a signal obtained by at least one surface selective analytical method for nitrogen which does not exceed 67 percent of that for a bulk amount of the crosslinked protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification