Alkali-treated bagasse, and its preparation and uses

• US 5,545,418 A
• Filed: 12/27/1994
• Issued: 08/13/1996
• Est. Priority Date: 12/27/1993
• Status: Expired due to Fees

• Current Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kaguku Kenkyujo
• Original Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kaguku Kenkyujo
• Inventors: Miyake, Toshio, Chaen, Hiroto, Iritani, Satoshi, Mitsuhashi, Masakazu
• Primary Examiner(s): Kepplinger, Esther M.
• Assistant Examiner(s): Koh, Choon P.
• Application Number:
  US08/364,489
• Time in Patent Office: 595 Days
• Field of Search: 426/53, 426/635, 426/61
• US Class Current: 426/53
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 10/32 from hydrolysates of wood or straw
• CPC Class Codes: A23V 2400/11 Lactobacillus
• CPC Class Codes: A23V 2400/21 Streptococcus, lactococcus
• CPC Class Codes: A23V 2400/41 Pediococcus
• CPC Class Codes: C05F 11/08 Organic fertilisers containing added bacterial cultures, mycelia or the like
• CPC Class Codes: C05F 5/002 Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
• CPC Class Codes: C05F 5/006 Waste from chemical processing of material, e.g. diestillation, roasting, cooking
• CPC Class Codes: C05F 5/008 Waste from biochemical processing of material, e.g. fermentation, breweries
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12R 2001/46 Streptococcus ; Enterococcus; Lactococcus
• CPC Class Codes: Y02A 40/20 Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
• CPC Class Codes: Y02P 20/145 the feedstock being materials of biological origin
• CPC Class Codes: Y02P 60/87 Re-use of by-products of food processing for fodder production
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales

Abstract

An alkali-treated bagasse is prepared by softening a bagasse with calcium oxide together with or without sodium hydroxide while preventing the substantial decomposition of cellulose and hemicellulose, a bagasse feed and a fermented bagasse feed prepared from the alkali-treated bagasse, and their preparations and uses as well as bacteria for fermenting the alkali-treated bagasse.

Claims

1. An alkali-treated bagasse, which is prepared by softening a bagasse with calcium oxide or with a mixture of calcium oxide and sodium hydroxide while preventing the substantial decomposition of cellulose and hemicellulose.

2. The alkali-treated bagasse of claim 1, wherein calcium oxide is incorporated into said bagasse together with or without sodium hydroxide under the coexistence of water.

3. The alkali-treated bagasse of claim 2, wherein the content of said water is in the range of about 40 to 90 w/w %.

4. The alkali-treated bagasse of claim 2, wherein said calcium oxide is incorporated into said bagasse in an amount of not exceeding 5 w/w % to said bagasse, on a dry solid basis.

5. The alkali-treated bagasse of claim 2, wherein said sodium hydroxide is incorporated into said bagasse in an amount of not exceeding the content of said calcium oxide.

6. The alkali-treated bagasse of claim 1, which is directly usable as a feed.

11. The alkali-treated bagasse of claim 1, which additionally contains a nutrient source.

13. A fermented bagasse feed obtained by incorporating a nutrient source and a seed culture of a lactic acid bacterium into said alkali-treated bagasse of claim 1;

and fermenting the resultant mixture.

14. The fermented bagasse feed of claim 13, wherein said seed culture is one or more members selected from the group consisting of lactic acid bacteria of the genera Enterococcus, Lactobacillus, Streptococcus and Pediococcus.

15. The fermented bagasse feed of claim 13, wherein said lactic acid bacterium of the genus Enterococcus is Enterococcus faecium FERM BP-4504.

16. The fermented bagasse feed of claim 13, wherein said nutrient source is a member selected from the group consisting of energy sources, proteins, minerals and/or vitamins.

17. The fermented bagasse feed of claim 13, wherein said nutrient source is incorporated thereinto in an amount not exceeding the amount of said bagasse, on a dry solid basis.

7. A process for preparing an alkali-treated bagasse, which consists essentially of;

(a) incorporating calcium oxide in an amount not exceeding 5% of the weight of said bagasse on a dry solid basis into a bagasse together with or without sodium hydroxide under the coexistence of water to subject said bagasse to a condition of not less than pH 10.3;

(b) allowing the mixture to stand; and

(c) recovering the resultant alkali-treated bagasse having a pH in the range of about 8 to less than 10.

8. The process of claim 7, wherein the content of said water is in the range of about 40 to 90 w/w %.

9. The process of claim 7, wherein said calcium oxide is incorporated into said bagasse in an amount of not exceeding 5 w/w % to said bagasse, on a dry solid basis.

12. The process of claim 7, wherein the step (c) further contains a step of incorporating a nutrient source into the resultant alkali-treated bagasse.

10. A process for softening a bagasse, which comprises incorporating calcium oxide into a bagasse together with or without sodium hydroxide into a bagasse under the coexistence of water to soften said bagasse while preventing the decomposition of cellulose and hemicellulose.

18. A process for preparing a fermented bagasse feed, which comprises:

(a) incorporating calcium oxide into a bagasse together with or without sodium hydroxide into a bagasse under the coexistence of water to subject said bagasse to a condition of not less than pH 10.3;

allowing the resultant mixture to stand;

(b) incorporating a nutrient source and a seed culture of a lactic acid bacterium into the resultant alkali-treated bagasse having a pH in the range of about 8 to less than 10; and

(c) fermenting the mixture and recovering the resultant fermented bagasse.

19. The process of claim 18, wherein said alkali-treated bagasse in the step of (b) is softened while preventing the substantial decomposition of cellulose and hemicellulose.

20. The process of claim 18, wherein said seed culture is one or more members selected from the group consisting of lactic acid bacteria of the genera Enterococcus, Lactobacillus, Streptococcus and Pediococcus.

21. The process of claim 20, wherein said lactic acid bacterium of the genus Enterococcus is a member selected from the group consisting of bacteria of the species Enterococcus casseliflavus, Enterococcus durans, Enterococcus faecalis, Enterococcus faecium, and Enterococcus hirae, and said lactic acid bacterium of the genus Lactobacillus is a member selected from the group consisting of bacteria of the species Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus sake, Lactobacillus acidophilus, Lactobacillus helveticus, and Lactobacillus brevis.