Electrolytic analytical methods
First Claim
1. Enzyme electrodes, having at least one active electrode component appropriate for the performance of an electrolytic measuring function, in association with an enzyme, wherein the enzyme is enclosed within liposomes and the liposomes, within which the enzyme is enclosed, are incorporated on or in a supporting carrier.
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Abstract
Enzyme electrodes having the enzyme enclosed within liposomes supported on, or in a membrane and immobilized therein are provided. The liposomes may be made using lipids and conveniently by "reverse phase evaporation". The enzyme electrodes incorporate conventional electrode elements, e.g., THE Clark electrode pair (Platinum/Silver) and additional membranes or screens, and used particularly in amperometric measurements. The enzyme can be an oxidase and is useful for measurement, directly or indirectly, of low molecular weight species generated by enzymic action, and effective for study of biological fluids.
25 Citations
15 Claims
- 1. Enzyme electrodes, having at least one active electrode component appropriate for the performance of an electrolytic measuring function, in association with an enzyme, wherein the enzyme is enclosed within liposomes and the liposomes, within which the enzyme is enclosed, are incorporated on or in a supporting carrier.
- 13. In a method for electro-analysis of solutions containing substances which interact with an enzyme in an enzyme electrode, the improvement which comprises enclosing the enzyme within liposomes.
Specification