Multifunctional nucleic acid monomer
First Claim
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1. A multifunctional nucleic acid monomer having the structurewhereinR1 is a base,stable acid-sensitive blocking group;
- R2 is a phosphorous derivative that enables addition of the nucleic acid to the 5'"'"'-position of an oligonucleotide chain during chemical synthesis;
R3 is selected from the group consisting of hydrogen, methyl, I, Br and F;
R4 is hydrogen or methyl; and
R5 is selected from the group consisting of levulinyl, ##STR20## in which R'"'"' is hydrogen, aryl or aralkyl;
the Ri may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy;
the Rj may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy;
the Rk may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy;
i is zero, 1, 2 or 3, j is zero, 1, 2, 3 or 4, and k is 0, 1, 2, 3 or 4; and
Z is selected from the group consisting of ##STR21## wherein x and y are integers which may be the same or different and are integers in the range of 1 to 8.
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Abstract
Novel reagents useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical phosphorylation of hydroxyl-containing compounds. The reagents are particularly useful for introducing cleavable sites and/or abasic sites into oligonucleotide or polynucleotide chains.
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1 Claim
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1. A multifunctional nucleic acid monomer having the structure
wherein R1 is a base,stable acid-sensitive blocking group; -
R2 is a phosphorous derivative that enables addition of the nucleic acid to the 5'"'"'-position of an oligonucleotide chain during chemical synthesis; R3 is selected from the group consisting of hydrogen, methyl, I, Br and F; R4 is hydrogen or methyl; and R5 is selected from the group consisting of levulinyl, ##STR20## in which R'"'"' is hydrogen, aryl or aralkyl;
the Ri may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy;the Rj may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy; the Rk may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy; i is zero, 1, 2 or 3, j is zero, 1, 2, 3 or 4, and k is 0, 1, 2, 3 or 4; and Z is selected from the group consisting of ##STR21## wherein x and y are integers which may be the same or different and are integers in the range of 1 to 8.
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Specification