Particle analyzer
First Claim
1. A particle analyzer for analyzing particles by passing a sheath liquid around a liquid specimen containing particles to be analyzed, and means for emitting light to the liquid specimen flow,the liquid specimen flow being a flat flow which is thin in thickness and broad in width,a one-dimensional image sensor disposed to extend in a direction vertical to the particle flow direction, and producing a signal at every scanning cycle for the particle, andsignal processing means for processing the signal from the one-dimensional image sensor, whereinthe signal processing means obtains feature parameters of individual particles on the basis of the signal from said one-dimensional image sensor, by processing the signal from the one dimensional image sensor, thereby analyzing the particles on the basis of the difference of the feature parameters of each particles, said feature parameters containing at least one of particle morphological information and absorption information,said signal processing means comprises:
- background correction processing means for obtaining correction data Sc(i), by calculating the difference between background data Sa obtained from the signal from the one-dimensional image sensor in the absence of a particle, and measured data Sb(i) obtained from the signal from the one-dimensional image sensor when a particle is passing through the imaging region,binarizing processing means for obtaining binary signal Sd(i) for detecting the specified portion of the particle by comparing the correction data Sc(i) from the background correction processing means with specified threshold data,binary signal processing means for logical operation for the binary signal from the binarizing processing means, andarithmetic means for calculating said feature parameters by using the binary signal from said binary signal processing means.
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Accused Products
Abstract
A particle analyzer comprising a one-dimensional image sensor for producing imaging signals by scanning a particle in a flow of specimen containing particle components such as blood and urine in a flat sheath flow thin in thickness in the light emitting direction and broad in the direction orthogonal to the light emitting direction, and a signal processing device for processing the signals and operating on the basis of the imaging signals from the one-dimensional image sensor. A particle analyzer excellent in analyzing capability for obtaining morphological information of particles in the liquid specimen flowing in a flow cell in real time is presented. Besides, by emitting light to a flowing particle, the transmitted light image is focused on the one-dimensional image sensor (line sensor), and the detected image signal from the line sensor is further processed in detail in the signal processing device. The signal processing device comprises a background correction processing circuit, a binarizing processing circuit, a binary signal processing circuit, a particle region division processing circuit and an arithmetic circuit. A particle analyzer high in analyzing capability for obtaining the absorption quantity and morphological information of individual particles in the liquid specimen flowing in a flow cell in real time is presented.
187 Citations
17 Claims
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1. A particle analyzer for analyzing particles by passing a sheath liquid around a liquid specimen containing particles to be analyzed, and means for emitting light to the liquid specimen flow,
the liquid specimen flow being a flat flow which is thin in thickness and broad in width, a one-dimensional image sensor disposed to extend in a direction vertical to the particle flow direction, and producing a signal at every scanning cycle for the particle, and signal processing means for processing the signal from the one-dimensional image sensor, wherein the signal processing means obtains feature parameters of individual particles on the basis of the signal from said one-dimensional image sensor, by processing the signal from the one dimensional image sensor, thereby analyzing the particles on the basis of the difference of the feature parameters of each particles, said feature parameters containing at least one of particle morphological information and absorption information, said signal processing means comprises: -
background correction processing means for obtaining correction data Sc(i), by calculating the difference between background data Sa obtained from the signal from the one-dimensional image sensor in the absence of a particle, and measured data Sb(i) obtained from the signal from the one-dimensional image sensor when a particle is passing through the imaging region, binarizing processing means for obtaining binary signal Sd(i) for detecting the specified portion of the particle by comparing the correction data Sc(i) from the background correction processing means with specified threshold data, binary signal processing means for logical operation for the binary signal from the binarizing processing means, and arithmetic means for calculating said feature parameters by using the binary signal from said binary signal processing means. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification